公开(公告)号：EP3170839A4

公开(公告)日：2018-01-03

申请号：EP15822054

申请日：2015-07-21

申请人： JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NAT INSTITUTE OF INFECTIOUS DISEASES ,  NIPPI INCORPORATED

发明人： SAITO SHINJI ,  SUZUKI TADAKI ,  HASEGAWA HIDEKI ,  AINAI AKIRA ,  GOTO KIYOKO ,  UENO TOMONORI ,  TAGA YUKI

IPC分类号： C07K16/18 ,  A61K39/395 ,  A61P31/00 ,  C07K16/10 ,  C12N15/09 ,  C12P21/08

CPC分类号： A61K39/395 ,  A61K2039/505 ,  A61K2039/507 ,  C07K16/065 ,  C07K16/10 ,  C07K16/18 ,  C12N15/01 ,  C12N15/09 ,  C12N15/1086 ,  C12N15/66

摘要： Provided are: a polymeric IgA-type recombinant antibody; a medicine containing this polymeric IgA-type recombinant antibody as an active ingredient; a method for producing this polymeric IgA type antibody, the method including the step of coexpressing an IgA-type antibody heavy-chain protein, an antibody light-chain protein, an antibody J-chain protein, and a secretory component protein within a single cell; and a method for improving the antigen-binding activity or neutralizing activity of this antibody, the method including the step of making an antibody into a polymeric IgA-type.

公开(公告)号：EP2990476A4

公开(公告)日：2017-05-03

申请号：EP14776222

申请日：2014-03-26

申请人： NIPPI INCORPORATED

发明人： UENO TOMONORI ,  TAGA YUKI ,  GOTO KIYOKO ,  KAKU YUKO

IPC分类号： C12N5/10 ,  A61K45/00 ,  A61K48/00 ,  A61P11/00 ,  A61P43/00 ,  C12N15/09 ,  C12P21/02

CPC分类号： C07K14/47 ,  C07K14/70553 ,  C12N15/67 ,  C12N15/85 ,  C12P21/00 ,  C12P21/02

摘要： It has been believed that promoting the assembly of polysomes composed of many ribosomes attached to mRNA is very effective for highly efficient protein synthesis. However, the mechanism for p180 protein's capability of promoting polysome formation has been yet to be elucidated. The inventors of the present application newly discovered SF3b4 protein as a protein that specifically interacts with the coiled-coil domain of p180 protein, a responsible region for its capability of promoting polysome formation, and which is capable of promoting mRNA localization to an endoplasmic reticulum (ER). The inventors also found that, in cells capable of highly expressing both p 180 protein and a protein promoting mRNA localization to an endoplasmic reticulum (ER) (e.g., SF3b4 protein), the mRNA localization to the endoplasmic reticulum can be significantly elevated so that the secretory capacity in cultured cells can be enhanced. Further, the inventors demonstrated that when a particular nucleotide sequence is inserted into an expression plasmid, SF3b4 protein exhibiting protein expression enhancing ability can be localized onto the endoplasmic reticulum membrane, and the mRNA distribution in polysomes can be shifted towards heavier fractions, whereby the secretory capacity in cells can be enhanced.

摘要翻译： 据认为，促进由连接到mRNA的许多核糖体组成的多核糖体组装对于高效蛋白质合成是非常有效的。 然而，p180蛋白促进多核糖体形成的能力的机制尚未阐明。 本申请的发明人新发现了SF3b4蛋白作为与p180蛋白的卷曲螺旋结构域特异性相互作用的蛋白质，p180蛋白是促进多核糖体形成的能力的责任区域，并且其能够促进mRNA定位至内质网 ER）。 发明人还发现，在能够高度表达p180蛋白和促进内质网（ER）的mRNA定位的蛋白（例如SF3b4蛋白）的细胞中，可以显着提高定位于内质网的mRNA，使得 可以提高培养细胞的分泌能力。 此外，本发明人证实，当将特定核苷酸序列插入表达质粒中时，表现出蛋白表达增强能力的SF3b4蛋白可定位于内质网膜上，并且多核糖体中的mRNA分布可朝向较重的级分移动，由此分泌 细胞容量可以增强。