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1.发明公开
公开(公告)号：EP1046717A9
公开(公告)日：2001-06-27
申请号：EP00108643
申请日：2000-04-20
发明人： KURANE RYUICHIRO , KANAGAWA TAKAHIRO , KAMAGATA YOICHI , KURATA SHINYA , YAMADA KAZUTAKA , YOKOMAKU TOYOKAZU , KOYAMA OSAMU , FURUSHO KENTA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q1/6837 , C12Q1/6851 , C12Q1/6858 , C12Q2565/549 , C12Q2565/107 , C12Q2563/107 , C12Q2525/185
摘要： A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises:
providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid;
hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.-
2.发明公开NOVEL NUCLEIC ACID PROBES AND METHOD OF ASSAYING NUCLEIC ACID BY USING THE SAME 有权用于核酸测试新的核酸探针和方法使用THEREOF
公开(公告)号：EP1295941A4
公开(公告)日：2004-06-16
申请号：EP01953279
申请日：2001-06-27
发明人： KANAGAWA TAKAHIRO , KURANE RYUICHIRO , YAMADA KAZUTAKA , YOKOMAKU TOYOKAZU , TORIMURA MASAKI , KAMAGATA YOICHI , KURATA SHINYA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6851 , Y02A90/26 , C12Q2565/107 , C12Q2565/1015
摘要： Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
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3.发明公开NOVEL NUCLEIC ACID PROBE AND NOVEL METHOD OF ASSAYING NUCLEIC ACIDS USING THE SAME 审中-公开新的核酸探针和核酸分析使用相同的新方法
公开(公告)号：EP1321520A4
公开(公告)日：2003-09-03
申请号：EP02713215
申请日：2002-03-27
发明人： KURANE RYUICHIRO , KANAGAWA TAKAHIRO , KAMAGATA YOICHI , TORIMURA MASAKI , KURATA SHINYA , YAMADA KAZUTAKA , YOKOMAKU TOYOKAZU
IPC分类号： C12Q1/68 , G01N33/58 , C12N15/09 , G01N33/53 , G01N33/566
CPC分类号： G01N33/582 , C12Q1/6818 , C12Q2565/101 , C12Q2565/107 , C12Q2565/1015
摘要： A novel nucleic acid probe for simultaneously assaying nucleic acids with a simple apparatus in case where one or more nucleic acids are present in the assay system, characterized in that, in a single-stranded nucleic acid probe labeled with a plural number of fluorescent pigments so as to form at least one pair of fluorescent pigments causing the FRET phenomenon (i.e., a fluorescent pigment capable of serving as a donor pigment and another fluorescent pigment capable of serving as an acceptor pigment), the base sequence is designed so that the fluorescent intensity of the acceptor pigment is weakened when the probe is hybridized with the target nucleic acids, and the above pigments are labeled.
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