公开(公告)号：EP1962093A4

公开(公告)日：2009-04-29

申请号：EP06833421

申请日：2006-11-27

申请人： JAPAN SCIENCE & TECH AGENCY ,  JAPAN ADV INST SCIENCE & TECH ,  BIO DEVICE TECHNOLOGIES CO LTD

发明人： NAGATANI NAOKI ,  YUI TERUKO ,  TAMIYA EIICHI ,  ENDOU TATSUROU ,  TANAKA RYOU

IPC分类号： G01N33/543

CPC分类号： G01N33/54393 ,  Y10S435/97

摘要： [PROBLEMS] To reduce the amount of, for example, a capturing antibody to be employed without lowering detection sensitivity. At the same time, to enable the achievement of intense color development or light emission in a determination area even in the case where only a small amount of a labeled antibody is accumulated. To lower the detection limit in the sandwich method. To enlarge the dynamic range in the competition method. [MEANS FOR SOLVING PROBLEMS] A method of analyzing a test substance by an immunological analysis method by using the test substance, a support having a determination area, on which one member selected from a capturing antibody capable of binding specifically to the test substance and a capturing antigen capable of binding specifically to the test substance has been immobilized, and a labeled antibody capable of binding specifically to the test substance, wherein a label having a sensitizing effect has been immobilized on the determination area of the support.