公开(公告)号：EP1909104A4

公开(公告)日：2009-03-04

申请号：EP06746921

申请日：2006-05-26

申请人： JAPAN SCIENCE & TECH AGENCY ,  NAT UNIV CORP KAGOSHIMA ,  SUDA YASUO

发明人： NISHIMURA TOMOAKI ,  KISHIMOTO YUKO ,  NAKAGAWA HIROMI ,  SUDA YASUO

IPC分类号： G01N33/543 ,  C07K1/22 ,  G01N33/553 ,  G01N33/58

CPC分类号： C07K17/14 ,  B82Y5/00 ,  C07K1/22 ,  G01N33/54346 ,  G01N33/553 ,  G01N33/587 ,  G01N2400/00

摘要： It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling.