公开(公告)号：EP2339339A4

公开(公告)日：2016-10-12

申请号：EP09816172

申请日：2009-09-24

申请人： JSR CORP

发明人： TAMORI KOUJI ,  FUKUTA TETSUO ,  MIYAJI MASAAKI ,  WANG YONG ,  ABE TAKAYOSHI ,  OKANO YUUSUKE ,  MOMIYAMA MASAKI ,  KAWAI TAKAHIRO

IPC分类号： G01N30/88 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31

CPC分类号： C07K1/22 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  Y10T428/29

摘要： An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.

公开(公告)号：EP2574631A4

公开(公告)日：2013-12-04

申请号：EP11759404

申请日：2011-03-23

申请人： JSR CORP

发明人： TAMORI KOUJI ,  FUKUTA TETSUO ,  MIYAJI MASAAKI ,  WANG YONG ,  ABE TAKAYOSHI ,  OKANO YUSUKE ,  MOMIYAMA MASAKI ,  KAWAI TAKAHIRO

IPC分类号： C07K17/08 ,  B01J20/32 ,  C07K1/22 ,  C07K19/00 ,  C12N15/09 ,  G01N30/88

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3219 ,  B01J20/3274 ,  C07K2317/10

摘要： Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒR-R 2 €ƒ€ƒ€ƒ€ƒ€ƒ(1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R 2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R 2 .