公开(公告)号：EP2982745A1

公开(公告)日：2016-02-10

申请号：EP14179989.0

申请日：2014-08-06

申请人： Johann Wolfgang Goethe-Universität, Frankfurt am Main

发明人： Ullrich, Evelyn ,  Gaetano, Carlo ,  Worm, Catharina ,  Cencioni, Chiara

IPC分类号： C12N5/078 ,  A61K35/14 ,  C12N5/0783

CPC分类号： C12N5/0634 ,  A61K35/15 ,  A61K35/17 ,  C12N5/0646 ,  C12N2501/065 ,  C12N2501/23

摘要： The present invention pertains to a method for the in-vitro or ex-vivo expansion of immune cells using a histone acetyl transferase (HAT) modulator as additive in the cell culture medium during the expansion procedure. Immune cells such as natural killer (NK) cells, T cells or cytokine induced killer (CIK) cells are used in adoptive cell therapies for the injection into patients suffering from malignant cancer diseases such as leukemia. Cells expanded using the method of the invention were superior proliferative and viable. Further provided by the invention is a cell culture medium comprising the HAT modulator of the invention as well as cell populations obtained using the method of the invention.

摘要翻译： 本发明涉及在扩增过程中，使用组蛋白乙酰转移酶（HAT）调节剂作为添加剂在细胞培养基中体外或离体扩增免疫细胞的方法。 免疫细胞如天然杀伤（NK）细胞，T细胞或细胞因子诱导的杀伤细胞（CIK）细胞被用于过继细胞疗法中，用于注射患有恶性癌症疾病例如白血病的患者。 使用本发明方法扩增的细胞具有优越的增殖和活力。 本发明进一步提供的是包含本发明的HAT调节剂的细胞培养基以及使用本发明的方法获得的细胞群。