公开(公告)号：EP4320218A1

公开(公告)日：2024-02-14

申请号：EP22720989.7

申请日：2022-04-05

申请人： Julius-Maximilians-Universität Würzburg

发明人： GROLL, Jürgen ,  RYMA, Matthias

IPC分类号： C12M3/00 ,  C12M1/12 ,  C12M1/00 ,  C12N5/00

公开(公告)号：EP4071234A1

公开(公告)日：2022-10-12

申请号：EP21167061.7

申请日：2021-04-06

申请人： Julius-Maximilians-Universität Würzburg

发明人： GROLL, Jürgen ,  RYMA, Matthias

IPC分类号： C12M3/00 ,  C12M1/12 ,  C12M1/00 ,  C12N5/00

摘要： The invention relates to a device for fabricating a perfusable three-dimensional tissue construct as well as to its manufacture and use. The device comprises a chamber in which the tissue construct may be cultivated, the chamber comprising an inlet and an outlet for perfusing the tissue construct, and a sacrificial scaffold fixed in the chamber. The sacrificial scaffold comprises a thermo-responsive polymer, the thermo-responsive polymer being not dissolvable in water at human body temperature and becoming dissolvable in water at a temperature below 30°C, preferably below 25°C. The sacrificial scaffold comprises at least one filament extending from the inlet to the outlet such that dissolution of the sacrificial scaffold provides for a liquid channel from the inlet to the outlet. The sacrificial scaffold seals the inlet and/or the outlet such that dissolution of the sacrificial scaffold after receiving a cultivation matrix in the chamber provides for a liquid channel from the inlet to the outlet through the cultivation matrix.