公开(公告)号：EP1621615A1

公开(公告)日：2006-02-01

申请号：EP04727416.2

申请日：2004-04-14

申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute

发明人： KOYANAGI, Satoshi, Chemo-Sero Therap. Res. Kikuchi ,  KAWATSU, Kenjiro, Chemo-Sero Therap. Res. Kikuchi ,  MURAKAMI, Toshio, Chemo-Sero Therap. Res. Kikuchi ,  MIYATSU, Yoshinobu, Chemo-Sero Therap. Res Kikuchi ,  MAEDA, Toshihiro, Chemo-Sero Therap. Res. Kikuchi ,  MIZOKAMI, Hiroshi, Chemo-Sero Therap. Res. Kikuchi

IPC分类号： C12N15/09 ,  C07K14/435 ,  A61K38/17 ,  A61P37/08

CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

摘要： A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

摘要翻译： 提供了通过遗传重组技术获得的改良的主要螨变应原的纯化方法和通过所述纯化方法获得的纯化的改性主要螨变应原。 一种用于纯化通过遗传重组技术获得的改性主要螨变应原的方法，其包括纯化步骤：（1）洗涤并回收含有通过MF膜的遗传重组技术获得的修饰的主要螨变应原的包含体; （2）溶解所述包涵体，然后重折叠; （3）浓缩含有改性主要螨变应原的溶液，同时用超滤膜除去低分子量组分; （4）用阴离子交换剂回收未吸附级分的改性主要螨变应原; （5）用疏水凝胶回收吸附级分中的改性主要螨变应原; 和（6）用阴离子交换剂回收吸附级分中的改性主要螨变应原，以及通过所述纯化方法获得的具有高纯度的改性主要螨变应原。