公开(公告)号：EP2546339B1

公开(公告)日：2016-09-07

申请号：EP11753494.1

申请日：2011-03-11

申请人： Kao Corporation

发明人： SHIMAZU, Ayako ,  ENDO, Keiji ,  OKUDA, Mitsuyoshi

IPC分类号： C12N9/42 ,  C11D3/386 ,  C11D7/42 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09

CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

公开(公告)号：EP3061817A1

公开(公告)日：2016-08-31

申请号：EP16163320.1

申请日：2010-04-26

申请人： KAO CORPORATION

发明人： TOHATA, Masatoshi ,  OKUDA, Mitsuyoshi ,  SATO, Tsuyoshi ,  ENDO, Keiji ,  SAEKI, Katsuhisa

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C11D3/38618 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/54

摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues:
(a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His;
(b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe;
(c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys;
(d) or a position corresponding thereto: Trp;
(e) or a position corresponding thereto: His, Trp, Ser, or Leu;
(f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, His, Lys, Arg, Met, or Asn;
(g) or a position corresponding thereto: Ala, Ser, or Cys;
(h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg;
(i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, His, or Ser; and
(j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.

摘要翻译： 由碱性蛋白酶衍生的碱性蛋白酶变体，其由SEQ ID NO：2所示的氨基酸序列组成或由与其同一性为90％以上的氨基酸序列组成，所述变体具有突变，其中一个或多个氨基酸残基 在从（a）位置6，（b）位置15，（c）位置16，（d）位置65，（e）位置66，（f）位置82，（g）位置83，（h） 204，（i）位置319和（j）由SEQ ID NO：2表示的氨基酸序列的位置337或与其相对应的位置被以下氨基酸残基取代：（a）或与其对应的位置： Typ，Leu，Val，Ile，Met，Tyr，Gln，Lys，Thr，Phe，Arg，Ser，Cys，Ala或His; （b）或对应位置：Glu，Met，Asp，Val，Gln，Arg，Cys，Trp，Ala或Phe; （c）或对应的位置：Met，Glu，Arg，Val，Lys，Phe，Tyr，Ile，His，Asp或Cys; （d）或对应的位置：Trp; （e）或与之对应的位置：His，Trp，Ser或Leu; （f）或对应位置：Ala，Glu，Gln，Ser，Cys，Gly，His，Lys，Arg，Met或Asn; （g）或对应的位置：Ala，Ser或Cys; （h）或对应的位置：Glu，Asp，Cys，Val，Thr，Pro，His，Ile，Trp，Ser，Asn，Lys或Arg; （i）或对应的位置：Trp，Val，Thr，Leu，Ile，Cys，Glu，Lys，Tyr，Arg，Phe，Gln，Met，Pro，Asp，Asn，His或Ser; 和（j）或对应的位置：Arg，Gly，Ser，Lys，Gln，Thr，His，Ala，Cys或Val。

公开(公告)号：EP2836594B1

公开(公告)日：2017-12-06

申请号：EP13718414.9

申请日：2013-04-09

申请人： Kao Corporation

发明人： TOHATA, Masatoshi ,  NISHIMURA, Yumi ,  WADA, Yasunao ,  SAEKI, Katsuhisa ,  OKUDA, Mitsuyoshi

IPC分类号： C11D3/386 ,  C12N9/54

CPC分类号： C12N9/54 ,  C11D3/38618

公开(公告)号：EP2546339A1

公开(公告)日：2013-01-16

申请号：EP11753494.1

申请日：2011-03-11

申请人： Kao Corporation

发明人： SHIMAZU, Ayako ,  ENDO, Keiji ,  OKUDA, Mitsuyoshi

IPC分类号： C12N9/42 ,  C11D3/386 ,  C11D7/42 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09

CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

摘要： The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.

摘要翻译： 本发明提供具有增强的抗再沉积能力的突变碱性纤维素酶。 本发明提供了一种突变碱性纤维素酶，其具有氨基酸序列，其中至少一个氨基酸残基选自对应于位置58,45,52,56,60,64,66,71,103， 119，122,123,124,125,127,130,140,​​161,164,175,176,178,179,181,193,194,195,196,197,199， 227,228，251，267，272，276，277，280，282，297，310，312，318，324，345，354，356，357，360，363，368，418，419，420，421， 422，453，454，455，457，458，459，494，495，496，500，501，502，503，504，550，551，552，604，605，606，607，608，640，641， 642，644，645，646，683，684，685，690，691，692，693，694，739，740，和碱性纤维素酶的氨基酸序列的741，在SEQ ID NO：2所示或所述 与SEQ ID NO：2的氨基酸序列具有至少70％同一性的碱性纤维素酶的氨基酸序列被其他氨基酸残基取代。

公开(公告)号：EP2115132A2

公开(公告)日：2009-11-11

申请号：EP08721976.2

申请日：2008-03-06

申请人： Kao Corporation

发明人： OKUDA, Mitsuyoshi

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C11D3/386 ,  C12N9/54

摘要： It is an object of the present invention to provide alkaline proteases having industrially sufficient protein productivity and a significant detergency. In the alkaline proteases, the amino acid residues at (a) position 9, (b) position 49, (c) position 194, (d) position 212, (e) position 237, (f) position 245, (g) position 281, (h) position 313, (i) position 379 and (j) position 427 in SEQ ID NO: 2 are selected from the following amino acid residues; Position (a); glutamine, Position (b); glutamine, Position (c); lysine or arginine, Position (d); arginine, asparagine or glutamine, Position (e); asparagines, Position (f ); asparagines. Position (g); arginine, Position (h); asparagines, Position (i); lysine, arginine, glutamic acid or aspartic acid, and Position (j); arginine.

公开(公告)号：EP3061817B1

公开(公告)日：2018-12-12

申请号：EP16163320.1

申请日：2010-04-26

申请人： KAO CORPORATION

发明人： TOHATA, Masatoshi ,  OKUDA, Mitsuyoshi ,  SATO, Tsuyoshi ,  ENDO, Keiji ,  SAEKI, Katsuhisa

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C11D3/38618 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/54

摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, His, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, His, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.

公开(公告)号：EP2836594A2

公开(公告)日：2015-02-18

申请号：EP13718414.9

申请日：2013-04-09

申请人： Kao Corporation

发明人： TOHATA, Masatoshi ,  NISHIMURA, Yumi ,  WADA, Yasunao ,  SAEKI, Katsuhisa ,  OKUDA, Mitsuyoshi

IPC分类号： C12N9/52 ,  C12N9/54

CPC分类号： C12N9/54 ,  C11D3/38618

摘要： Providing an alkaline protease exhibiting an improved solubility in a liquid detergent. A mutant alkaline protease consisting of the amino acid sequence represented by SEQ ID No: 2 or an amino acid sequence having 80% or more sequence identity therewith, in which at least one amino acid residue selected from the group consisting of the amino acid residues at predetermined positions of the amino acid sequence represented by SEQ ID No: 2 or corresponding positions thereto are substituted.

摘要翻译： 提供在液体洗涤剂中表现出改善的溶解度的碱性蛋白酶。 由SEQ ID No：2所示的氨基酸序列或与其序列同一性为80％以上的氨基酸序列组成的突变型碱性蛋白酶，其中至少一个选自下列氨基酸残基的氨基酸残基： 由SEQ ID No：2表示的氨基酸序列或其对应位置的预定位置被取代。

公开(公告)号：EP2546339A9

公开(公告)日：2013-07-03

申请号：EP11753494.1

申请日：2011-03-11

申请人： Kao Corporation

发明人： SHIMAZU, Ayako ,  ENDO, Keiji ,  OKUDA, Mitsuyoshi

IPC分类号： C12N9/42 ,  C11D3/386 ,  C11D7/42 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09

CPC分类号： C11D3/38645 ,  C12N9/2437 ,  C12Y302/01004

摘要： The present invention provides a mutant alkaline cellulase having enhanced anti-redeposition ability. The present invention provides a mutant alkaline cellulase having an amino acid sequence in which at least amino acid residue selected from the amino acid residues at the positions corresponding to positions 58, 45, 52, 56, 60, 64, 66, 71, 103, 119, 122, 123, 124, 125, 127, 130, 140, 161, 164, 175, 176, 178, 179, 181, 193, 194, 195, 196, 197, 199, 202, 203, 217, 225, 227, 228, 251, 267, 272, 276, 277, 280, 282, 297, 310, 312, 318, 324, 345, 354, 356, 357, 360, 363, 368, 418, 419, 420, 421, 422, 453, 454, 455, 457, 458, 459, 494, 495, 496, 500, 501, 502, 503, 504, 550, 551, 552, 604, 605, 606, 607, 608, 640, 641, 642, 644, 645, 646, 683, 684, 685, 690, 691, 692, 693, 694, 739, 740, and 741 of the amino acid sequence of an alkaline cellulase set forth in SEQ ID NO: 2 or of the amino acid sequence of an alkaline cellulase having at least 70% identity with the amino acid sequence of SEQ ID NO: 2, is substituted with other amino acid residue.

公开(公告)号：EP2424981A2

公开(公告)日：2012-03-07

申请号：EP10719131.4

申请日：2010-04-26

申请人： Kao Corporation

发明人： TOHATA, Masatoshi ,  OKUDA, Mitsuyoshi ,  SATO, Tsuyoshi ,  ENDO, Keiji ,  SAEKI, Katsuhisa

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C11D3/38618 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/54

摘要： An alkaline protease variant derived from an alkaline protease consisting of an amino acid sequence represented by SEQ ID NO: 2 or consisting of an amino acid sequence having an identity of 90% or more therewith, which variant has mutations wherein one or more amino acid residues at positions selected from (a) position 6, (b) position 15, (c) position 16, (d) position 65, (e) position 66, (f) position 82, (g) position 83, (h) position 204, (i) position 319, and (j) position 337 of the amino acid sequence represented by SEQ ID NO: 2, or at positions corresponding thereto are substituted with the following amino acid residues: (a) or a position corresponding thereto: Typ, Leu, Val, Ile, Met, Tyr, Gln, Lys, Thr, Phe, Arg, Ser, Cys, Ala, or His; (b) or a position corresponding thereto: Glu, Met, Asp, Val, Gln, Arg, Cys, Trp, Ala, or Phe; (c) or a position corresponding thereto: Met, Glu, Arg, Val, Lys, Phe, Tyr, Ile, His, Asp, or Cys; (d) or a position corresponding thereto: Trp; (e) or a position corresponding thereto: His, Trp, Ser, or Leu; (f) or a position corresponding thereto: Ala, Glu, Gln, Ser, Cys, Gly, His, Lys, Arg, Met, or Asn; (g) or a position corresponding thereto: Ala, Ser, or Cys; (h) or a position corresponding thereto: Glu, Asp, Cys, Val, Thr, Pro, His, Ile, Trp, Ser, Asn, Lys, or Arg; (i) or a position corresponding thereto: Trp, Val, Thr, Leu, Ile, Cys, Glu, Lys, Tyr, Arg, Phe, Gln, Met, Pro, Asp, Asn, His, or Ser; and (j) or a position corresponding thereto: Arg, Gly, Ser, Lys, Gln, Thr, His, Ala, Cys, or Val.