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    Bioluminescence reagent and method for quantitative determination of adenosine phosphate ester using the reagent
    1.
    发明公开
    Bioluminescence reagent and method for quantitative determination of adenosine phosphate ester using the reagent 失效
    Bioluminiszenzreagens和方法，用于使用该试剂Andenosinphosphatestern的定量测定

    公开(公告)号：EP0794260A1

    公开(公告)日：1997-09-10

    申请号：EP97103430.1

    申请日：1997-03-03

    申请人： KIKKOMAN CORPORATION

    发明人： Murakami, Seiji ,  Sakakibara, Tatsuya ,  Eisaki, Naoki ,  Nakajima, Motoo ,  Imai, Kazuhiro

    IPC分类号： C12Q1/66 ,  C12Q1/48

    CPC分类号： C12Q1/48 ,  C12Q1/66

    摘要： There is provided a bioluminescence reagent comprising at least pyruvate orthophosphate dikinase, phosphoenolpyruvic acid, pyrophosphoric acid, magnesium ion or another metallic ions, luciferin and luciferase, which reagent is such that the amount of luminescence is maintained in a high level and moreover stably without decaying for a long time in a bioluminescence reaction, and there is provided a method for quantitatively determining an adenosine phosphate ester or a substance taking part in the ATP conversion reaction in high sensitivity and high accuracy using an inexpensive and simple measuring apparatus.

    摘要翻译： 提供了一种生物发光试剂，其包含至少丙酮酸正磷酸二激酶，磷酸烯醇式丙酮酸，焦磷酸，镁离子或其它金属离子，萤光素和萤光素酶，其中试剂被检查做发光量保持在较高的水平，更超过平稳，无衰减 用于在生物发光反应时间长，且有对腺苷磷酸酯或使用在便宜和简单的测量装置，在高灵敏度和高准确度的ATP转化反应的物质参与的定量确定性采矿的方法。