专利检索 ap:("KINEMED, INC.") AND inv:"ANGEL, Thomas E." 第 1 页

1.

发明公开
MEASUREMENT OF MOLECULAR FLUX RATES BY QUANTIFYING ISOTOPOLOGUE ABUNDANCES USING HIGH RESOLUTION MASS SPECTROMETRY 审中-公开

公开(公告)号：EP3347718A1

公开(公告)日：2018-07-18

申请号：EP16844986.6

申请日：2016-09-07

申请人： KINEMED, INC.

发明人： HELLERSTEIN, Marc K. , ANGEL, Thomas E.

IPC分类号： G01N33/58

CPC分类号： G01N33/58 , G01N33/6848 , G01N2458/15

摘要： Provided herein are methods for measuring a molecular flux rate based on analysis of isotopologue abundance within a mass isotopomer, e.g., using a high resolution mass spectrometric measurement. Such methods may be used, inter alia, to calculate a fraction of newly synthesized target molecules of interest, a replacement rate of target molecules of interest, and/or a rate of breakdown or degradation of target molecules of interest, e.g., based on isotopologue relative abundance.

2.

发明公开
MEASUREMENT OF PROTEIN MOLECULAR FLUX RATES BY QUANTIFYING ISOTOPOLOGUE ABUNDANCES IN IMMONIUM ION FRAGMENTS USING HIGH RESOLUTION MASS SPECTROMETRY 审中-公开

公开(公告)号：EP3347713A2

公开(公告)日：2018-07-18

申请号：EP16844988.2

申请日：2016-09-07

申请人： KINEMED, INC.

发明人： HELLERSTEIN, Marc K. , ANGEL, Thomas E. , MAKAROV, Alexander A.

IPC分类号： G01N33/50 , G01N33/58 , G01N33/68

CPC分类号： G06F19/20 , G01N33/6848

摘要： Provided herein are methods for measuring a molecular flux rate proteins and polypeptides based on analysis of isotopologue abundances of immonium ion fragments within a mass isotopomer using a high resolution mass spectrometric measurement. Such methods may be used, inter alia, to calculate a fraction of newly synthesized protein or polypeptide molecules of interest, a replacement rate of target molecules of interest, and/or a rate of breakdown or degradation of target molecules of interest, e.g., based on isotopologue relative abundances.