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    Preparation of proliferation inhibitors
    2.
    发明公开
    Preparation of proliferation inhibitors 失效
    Herstellung von Proliferationsinhibitoren。

    公开(公告)号：EP0136053A2

    公开(公告)日：1985-04-03

    申请号：EP84305664.9

    申请日：1984-08-20

    申请人： KOKEN LTD. ,  Tajima, Tomoyuki ,  Nagasu, Youichiro

    发明人： Tajima, Tomoyuki

    IPC分类号： C12P1/00 ,  C12N5/00

    CPC分类号： C07K14/4703 ,  A61K38/00 ,  C12P1/00 ,  C12R1/91

    摘要： This invention relates to a method for preparing a proliferation inhibitor for malignant tumour cells of animals including human beings, and the method comprises the steps of cultivating the malignant tumour cells of the animals, afterward transferring them to a culture medium for extraction, adding glucose to the medium, carrying out a cultivation therein, afterward removing the malignant tumour cells therefrom, and collecting the remaining culture medium.

    摘要翻译： 本发明涉及一种制备包括人类在内的动物恶性肿瘤细胞增殖抑制剂的方法，该方法包括以下步骤：培养动物的恶性肿瘤细胞，然后将其转移到培养基中进行提取，将葡萄糖加入 该培养基进行培养，然后从其中除去恶性肿瘤细胞，并收集剩余的培养基。

    An agent for inhibiting the proliferation of malignant tumour cells and a process for the preparation thereof
    3.
    发明公开
    An agent for inhibiting the proliferation of malignant tumour cells and a process for the preparation thereof 失效
    用于抑制恶性肿瘤细胞增殖的药剂及其制备方法

    公开(公告)号：EP0125125A3

    公开(公告)日：1986-10-08

    申请号：EP84303035

    申请日：1984-05-04

    申请人： KOKEN LTD. ,  Tajima, Tomoyuki ,  Nagasu, Youichiro

    发明人： Tajima, Tomoyuki

    IPC分类号： C12P01/00 ,  C12N05/00 ,  A61K35/12 ,  C12R01/91

    CPC分类号： A61K35/13 ,  C12P1/00 ,  C12R1/91

    摘要： Agents for inhibiting the proliferation of malignant tumour cells are described together with processes for their preparation. Malignant tumour cells are cultivated in a culture medium for a period before being removed therefrom. By means of fractional filtration from the culture medium, that fraction having a molecular weight of not less than 500 is separated. Results show that this fraction has a significant inhibiting effect against proliferation of malignant tumour cells.

    摘要翻译： 与其制备方法一起描述用于抑制恶性肿瘤细胞增殖的药剂。 将恶性肿瘤细胞在培养基中培养一段时间，然后从其中除去。 通过从培养基中分级过滤，分离分子量不小于500的级分。 结果表明，该组分对恶性肿瘤细胞增殖具有显着的抑制作用。

    An agent for inhibiting the proliferation of malignant tumour cells and a process for the preparation thereof
    5.
    发明公开
    An agent for inhibiting the proliferation of malignant tumour cells and a process for the preparation thereof 失效
    装置，用于抑制恶性肿瘤细胞的增殖，和方法及其制备。

    公开(公告)号：EP0125125A2

    公开(公告)日：1984-11-14

    申请号：EP84303035.4

    申请日：1984-05-04

    申请人： KOKEN LTD. ,  Tajima, Tomoyuki ,  Nagasu, Youichiro

    发明人： Tajima, Tomoyuki

    IPC分类号： C12P1/00 ,  C12N5/00 ,  A61K35/12

    CPC分类号： A61K35/13 ,  C12P1/00 ,  C12R1/91

    摘要： Agents for inhibiting the proliferation of malignant tumour cells are described together with processes for their preparation. Malignant tumour cells are cultivated in a culture medium for a period before being removed therefrom. By means of fractional filtration from the culture medium, that fraction having a molecular weight of not less than 500 is separated. Results show that this fraction has a significant inhibiting effect against proliferation of malignant tumour cells.

    摘要翻译： 用于抑制恶性肿瘤细胞的增殖剂与它们的制备方法一起描述。 恶性肿瘤细胞在一段的培养基被去除之前存在栽培。 通过从培养基中分数过滤手段，没有具有不小于500的分子量的级分分离。 结果表明thatthis级分具有抗抑制恶性肿瘤细胞增殖的显著效果。

    A process for producing inhibiting agents against proliferation of malignant tumour cells, and agents produced thereby
    10.
    发明公开
    A process for producing inhibiting agents against proliferation of malignant tumour cells, and agents produced thereby 失效
    一种用于制备恶性癌细胞，抑制剂，以及由此制得的试剂的扩散过程。

    公开(公告)号：EP0133029A2

    公开(公告)日：1985-02-13

    申请号：EP84305099.8

    申请日：1984-07-26

    申请人： KOKEN LTD. ,  Tajima, Tomoyuki ,  Nagasu, Youichiro

    发明人： Tajima, Tomoyuki

    IPC分类号： A61K35/12

    CPC分类号： C07K14/4703 ,  A61K38/00 ,  C12P1/00 ,  C12R1/91

    摘要： An inhibiting agent against the proliferation of malignant tumour cells is produced by first incubating malignanttumour cells, and then further incubating the malignant tumour cells after adding serum protein or albumin to the culture medium. The medium is isolated by removing the cells, and is then subjected to ultra-fiftration through a filter for the molecular weight of 10,000. The fraction having a molecular weight of 10,000 or more is separated.

    摘要翻译： 上抑制剂对恶​​性肿瘤细胞的增殖是通过首先孕育恶性肿瘤细胞，然后加入血清蛋白或白蛋白到培养基中之后进一步孵育恶性肿瘤细胞中产生。 该介质通过去除细胞中分离，然后通过为10,000分子量的过滤器进行超滤。 具有10,000或更大的分子量的级分分离。