公开(公告)号：EP1226752A1

公开(公告)日：2002-07-31

申请号：EP00971708.3

申请日：2000-11-01

申请人： KYOWA HAKKO KOGYO CO., LTD.

发明人： MORI, Katsuhiro, Kyowa Hakko Kogyo Ltd ,  SATO, Mitsuo, Kyowa Hakko Kogyo Ltd ,  SEKINE, Susumu, Kyowa Hakko Kogyo Ltd

IPC分类号： A01K67/027 ,  G01N33/50 ,  G01N33/15 ,  C12N5/10 ,  C12N15/09 ,  C12N15/63 ,  C12Q1/02

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/0393 ,  C07K14/43595 ,  C12N15/8509 ,  C12N2800/30

摘要： The present invention provides a transgenic non-human mammal which has DNA encoding a foreign reporter protein for monitoring changes in intracellular calcium ion concentration and expresses the reporter protein in one or more types of non-neuronal cells; a method for producing the above transgenic non-human mammal; a method for detecting changes in intracellular calcium ion concentration using the above transgenic non-human mammal; DNA having promoter activity which is used for production of the transgenic non-human mammal; and the method of use of the DNA. The non-human mammal of the present invention expresses the DNA in one or more tissues or organs selected from the group consisting of epithelial tissue, connective tissue, adipose tissue, cartilage tissue, bone tissue, muscle tissue, intraoral tissue, blood tissue, bone marrow, cardiovascular organ, lymphatic organ, teeth, digestive tractl, liver, gallbladder, pancreas, respiratory organ, urinary organ, genital organ, endocrine organ, sense organ and nail, and can be used for evaluation of pharmacological effect and activity of physiologically active substances (for example, drug), novel gene products having unknown functions and the like in these tissues or organs.

摘要翻译： 本发明提供一种转基因非人哺乳动物其中有DNA编码外源报告蛋白用于监测细胞内钙离子浓度和在一个或多个类型的非神经元细胞的快车报告蛋白的变化; 生产上述的转基因非人类哺乳动物的方法; 为了检测使用上述转基因非人类哺乳动物的细胞内钙离子浓度的变化的方法; 具有启动子活性的所有其用于生产转基因的非人哺乳动物的DNA; 和使用DNA的方法。 本发明的非人类哺乳动物快车的DNA在选自上皮组织，结缔组织，脂肪组织，软骨组织，骨组织，肌肉组织，口腔内组织，血液组织，骨中选择的一个或多个组织或器官 骨髓，心血管器官，淋巴器官，牙齿，消化道tractl，肝，胆，胰，呼吸器官，泌尿器官，生殖器官，内分泌器官，感觉器官和指甲，以及可用于生理活性的药理作用和活性评价 物质（例如，药物），新颖的基因产物具有未知功能和在论文组织或器官等。