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公开(公告)号：EP1666476B1
公开(公告)日：2008-01-16
申请号：EP05024213.0
申请日：2000-03-03
发明人： Honda, Shinkichi , Takahashi, Tomoya c/o Kyowa Hakko Kogyo Co.,Ltd. , Kamimura, Ayako c/o Kyowa Hakko Kogyo Co.,Ltd. , Matsuoka, Takako , Kanda, Tomomasa c/o Asahi Breweries, Ltd. , Yanagida, Akio , Hieda, Kazuo
IPC分类号： C07D311/60 , C07D311/62 , C12P17/06
CPC分类号： C07D311/62 , C07D311/60
摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.
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2.发明公开A process for purification of proanthocyanidin oligomers 有权Verfahren zur Reinigung von Proanthocyanidin-Oligomeren
公开(公告)号：EP1666476A1
公开(公告)日：2006-06-07
申请号：EP05024213.0
申请日：2000-03-03
发明人： Honda, Shinkichi , Takahashi, Tomoya c/o Kyowa Hakko Kogyo Co.,Ltd. , Kamimura, Ayako c/o Kyowa Hakko Kogyo Co.,Ltd. , Matsuoka, Takako , Kanda, Tomomasa , Yanagida, Akio , Hieda, Kazuo
IPC分类号： C07D311/60 , C07D311/62 , C12P17/06
CPC分类号： C07D311/62 , C07D311/60
摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.
摘要翻译： 本发明涉及一种具有均匀聚合度的二聚至五聚原花青素低聚物的纯化方法，该方法包括通过通过正相硅胶液相色谱法分离和纯化原花青素低聚物的原料通过聚合度从原花青素低聚物或粗提纯产物中分离纯化 作为流动相，可以使用选自酯溶剂，酮溶剂，烃溶剂，醚溶剂和醇溶剂的两种或更多种溶剂的单一溶剂或混合溶剂。
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公开(公告)号：EP1632486A3
公开(公告)日：2006-03-15
申请号：EP05024212.2
申请日：2000-03-03
发明人： Honda, Shinkichi , Takahashi, Tomoya c/o Kyowa Hakko Kogyo Co.,Ltd. , Kamimura, Ayako c/o Kyowa Hakko Kogyo Co.,Ltd. , Matsuoka, Takako , Kanda, Tomomasa , Yanagida, Akio , Hieda, Kazuo
IPC分类号： C07D311/60 , C07D311/62 , C12P17/06
CPC分类号： C07D311/62 , C07D311/60
摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these.
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公开(公告)号：EP1632486A2
公开(公告)日：2006-03-08
申请号：EP05024212.2
申请日：2000-03-03
发明人： Honda, Shinkichi , Takahashi, Tomoya c/o Kyowa Hakko Kogyo Co.,Ltd. , Kamimura, Ayako c/o Kyowa Hakko Kogyo Co.,Ltd. , Matsuoka, Takako , Kanda, Tomomasa , Yanagida, Akio , Hieda, Kazuo
IPC分类号： C07D311/60 , C07D311/62 , C12P17/06
CPC分类号： C07D311/62 , C07D311/60
摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these.
摘要翻译： 本发明涉及一种纯化二聚体至五聚体原花青素低聚物的方法，其包括用含有原花青素低聚物的水解原料的酶进行预处理，从其中纯化粗产物或含有这些之一的溶液。
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公开(公告)号：EP1632486B1
公开(公告)日：2008-05-28
申请号：EP05024212.2
申请日：2000-03-03
发明人： Honda, Shinkichi , Takahashi, Tomoya c/o Kyowa Hakko Kogyo Co.,Ltd. , Kamimura, Ayako c/o Kyowa Hakko Kogyo Co.,Ltd. , Matsuoka, Takako , Kanda, Tomomasa c/o Asahi Breweries, Ltd. , Yanagida, Akio , Hieda, Kazuo Frontier Food Ltd.
IPC分类号： C07D311/60 , C07D311/62 , C12P17/06
CPC分类号： C07D311/62 , C07D311/60
摘要： The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.
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