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公开(公告)号：EP0272064B1
公开(公告)日：1993-03-10
申请号：EP87310938.3
申请日：1987-12-11
发明人： Fujio, Tatsuro , Maruyama, Akihiko
IPC分类号： C12P17/04
CPC分类号： C12P17/04
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2.发明公开
公开(公告)号：EP0263716A3
公开(公告)日：1988-12-28
申请号：EP87308957
申请日：1987-10-09
摘要： 5ʹ-guanylic acid may be produced with higher yield by an enzymatic reaction using, as a source of enzyme, fermented product such as fermented liquor and microbial cells obtained by culturing a transformant of Escherichia coli which is capable of producing 5ʹ-guanylic acid from 5ʹ-xanthylic acid, adenosine-5ʹ-triphosphate and at least one substrate selected from ammonia and glutamine and which is produced by transformation of a strain of E. coli carrying a temperature-sensitive cl repressor with a novel recombinant plasmid prepared by recombination of a DNA fragment carrying the P L promoter located upstream of a gene of 5ʹ-guanylic acid synthetase with a vector DNA. It is possible to culture the transformed microorganism in a medium containing 5ʹ-xanthylic acid, adenosine-5ʹ-triphosphate and at least one member selected from ammonia and glutamine.
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公开(公告)号：EP0319130A3
公开(公告)日：1990-05-16
申请号：EP88309279.3
申请日：1988-10-05
摘要： A process for the preparation of ascorbic acid-2-phosphate, which comprises reacting ascorbic acid or araboascorbic acid with a phosphate donor other than ATP in an aqueous medium in the presence of an effective amount of an enzyme derived and capable of preferentially catalyzing the enzymatic phosphorylation in the 2-position of ascorbic acid or araboascorbic acid by said phosphate donor, and recovering the resultant ascorbic acid-2-phosphate from the reaction solution.
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4.发明公开Process for the preparation of ascorbic acid-2-phosphate 失效Verfahren zur Herstellung vonAscorbinsäure-2-phosphat。
公开(公告)号：EP0319130A2
公开(公告)日：1989-06-07
申请号：EP88309279.3
申请日：1988-10-05
摘要： A process for the preparation of ascorbic acid-2-phosphate, which comprises reacting ascorbic acid or araboascorbic acid with a phosphate donor other than ATP in an aqueous medium in the presence of an effective amount of an enzyme derived and capable of preferentially catalyzing the enzymatic phosphorylation in the 2-position of ascorbic acid or araboascorbic acid by said phosphate donor, and recovering the resultant ascorbic acid-2-phosphate from the reaction solution.
摘要翻译： 一种制备抗坏血酸-2-磷酸酯的方法，其包括在水性介质中，在有效量的酶存在下，使抗坏血酸或阿拉伯糖酸与除ATP以外的磷酸盐供体反应，并能够优先催化酶 通过所述磷酸盐供体在抗坏血酸或阿糖抗坏血酸的2位上磷酸化，并从反应溶液中回收得到的抗坏血酸-2-磷酸。
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公开(公告)号：EP0319130B1
公开(公告)日：1994-03-09
申请号：EP88309279.3
申请日：1988-10-05
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6.发明公开A process for the preparation of ascorbic acid-2-phosphate 失效Verfahren zur Herstellung vonAscorbinsäure-2-phosphat。
公开(公告)号：EP0272064A2
公开(公告)日：1988-06-22
申请号：EP87310938.3
申请日：1987-12-11
发明人： Fujio, Tatsuro , Maruyama, Akihiko
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： The invention relates to a process for the preparation of ascorbic acid-2-phosphate, which comprises reacting ascorbic acid or araboascorbic acid with ATP to produce ascorbic acid-2-phosphate in an aqueous medium in the presence of an effective amount of an enzyme derived from a microorganism and capable of catalyzing the enzymatic reaction of ascorbic acid or araboascorbic acid with ATP to produce ascorbic acid-2-phosphate and recovering the resultant ascorbic acid-2-phosphate from the reaction solution.
摘要翻译： 本发明涉及一种制备抗坏血酸-2-磷酸的方法，其包括使抗坏血酸或阿拉伯糖酸与ATP反应，以在有效量的酶存在下在水性介质中产生抗坏血酸-2-磷酸 并且能够用ATP催化抗坏血酸或阿拉伯糖酸的酶促反应以产生抗坏血酸-2-磷酸，并从反应溶液中回收得到的抗坏血酸-2-磷酸。
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7.发明公开A process for the preparation of 5'-guanylic acid and expression vectors for use therein 失效一种生产5'-guanilsäure和表达过程矢量于此用途。
公开(公告)号：EP0263716A2
公开(公告)日：1988-04-13
申请号：EP87308957.7
申请日：1987-10-09
摘要： 5ʹ-guanylic acid may be produced with higher yield by an enzymatic reaction using, as a source of enzyme, fermented product such as fermented liquor and microbial cells obtained by culturing a transformant of Escherichia coli which is capable of producing 5ʹ-guanylic acid from 5ʹ-xanthylic acid, adenosine-5ʹ-triphosphate and at least one substrate selected from ammonia and glutamine and which is produced by transformation of a strain of E. coli carrying a temperature-sensitive cl repressor with a novel recombinant plasmid prepared by recombination of a DNA fragment carrying the P L promoter located upstream of a gene of 5ʹ-guanylic acid synthetase with a vector DNA. It is possible to culture the transformed microorganism in a medium containing 5ʹ-xanthylic acid, adenosine-5ʹ-triphosphate and at least one member selected from ammonia and glutamine.
摘要翻译： 5“鸟苷酸可以用更高的产量利用生产通过在酶反应，作为酶的来源，发酵产品：如发酵液和通过培养大肠杆菌的转化体而得到的微生物细胞的所有其能够产生5'-鸟苷酸的 从5'-黄苷酸，腺苷-5'-三磷酸和由氨和谷氨酰胺中的至少一种底物和所有其由携带温度敏感性C1阻遏物与由制得的新的重组质粒的大肠杆菌菌株的转化产生 携带位于的5'-鸟苷酸合成酶与载体DNA的基因的上游的PL启动子的DNA片段的重组。 是可能的培养在含有5'-黄苷酸，从氨和谷氨酰胺选择腺苷-5'-三磷酸和至少一个部件上的介质中的转化的微生物。
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公开(公告)号：EP0272064A3
公开(公告)日：1989-06-07
申请号：EP87310938.3
申请日：1987-12-11
发明人： Fujio, Tatsuro , Maruyama, Akihiko
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： The invention relates to a process for the preparation of ascorbic acid-2-phosphate, which comprises reacting ascorbic acid or araboascorbic acid with ATP to produce ascorbic acid-2-phosphate in an aqueous medium in the presence of an effective amount of an enzyme derived from a microorganism and capable of catalyzing the enzymatic reaction of ascorbic acid or araboascorbic acid with ATP to produce ascorbic acid-2-phosphate and recovering the resultant ascorbic acid-2-phosphate from the reaction solution.
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