公开(公告)号：EP1609868B1

公开(公告)日：2015-11-11

申请号：EP04703505.0

申请日：2004-01-20

申请人： Kaneka Corporation

发明人： YANAGITA, Yoshifumi 1-701, Saususukuea ,  OGAWA, Noriko ,  UEDA, Yasuyoshi ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62 ,  C08G63/89

CPC分类号： C12P7/625

公开(公告)号：EP1609868A1

公开(公告)日：2005-12-28

申请号：EP04703505.0

申请日：2004-01-20

申请人： KANEKA CORPORATION

发明人： YANAGITA, Yoshifumi 1-701, Saususukuea ,  OGAWA, Noriko ,  UEDA, Yasuyoshi ,  OSAKADA, Fumio ,  MATSUMOTO, Keiji

IPC分类号： C12P7/62

CPC分类号： C12P7/625

摘要： The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.
The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.

摘要翻译： 本发明的目的是提供一种分离纯化PHA的方法，而不会导致分子量的严重降低以获得高产率的高纯度PHA，其包括从培养的PHA有效地除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的用于回收PHA的方法是一种方法，其包括通过在低温下进行物理破坏处理和碱添加来有效地破坏细胞以恢复PHA，以获得PHA的水性悬浮液， 含有微生物细胞，然后用酶和/或表面活性剂处理PHA。 此外，通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集，可以扩大PHA的粒径。