公开(公告)号：EP4386082A1

公开(公告)日：2024-06-19

申请号：EP22855868.0

申请日：2022-08-08

申请人： Kaneka Corporation ,  RIKEN

发明人： KAMBAYASHI, Sho ,  KAWAI, Yoshikazu ,  HAYASHI, Yohei ,  TAKASAKI, Mami

IPC分类号： C12N5/074 ,  C12N5/0735

CPC分类号： C12N5/06

摘要： The death of some pluripotent stem cells in suspension culture of pluripotent stem cells is avoided. When performing the suspension culture of the pluripotent stem cells, a liquid medium that does not substantially contain a PKCβ inhibitor is used for a single-cell state and a liquid medium containing a PKCβ inhibitor and a WNT inhibitor is used after a cell mass is formed.

公开(公告)号：EP4105319A1

公开(公告)日：2022-12-21

申请号：EP21754008.7

申请日：2021-02-12

申请人： Kaneka Corporation ,  RIKEN

发明人： TAKEUCHI, Kazuhiro ,  IBUKI, Masato ,  HAYASHI, Yohei ,  TAKASAKI, Mami ,  KAMBAYASHI, Sho ,  KAWAI, Yoshikazu

IPC分类号： C12N5/0735 ,  C12N5/10 ,  C12N9/99

摘要： Pluripotent stem cells are suspension-cultured with the undifferentiated state thereof maintained. In suspension culture of pluripotent stem cells, the undifferentiated state is maintained by the presence of a PKC inhibitor, especially, a PKCβ inhibitor, and a tankyrase inhibitor (TNKS inhibitor).

公开(公告)号：EP4257678A1

公开(公告)日：2023-10-11

申请号：EP21903397.4

申请日：2021-12-07

申请人： Kaneka Corporation ,  RIKEN

发明人： KAMBAYASHI, Sho ,  HAYASHI, Yohei ,  TAKASAKI, Mami

IPC分类号： C12N5/10 ,  C12N15/09

摘要： This invention is intended to suppress cell death occurring at the time of transition of adherent culture of pluripotent stem cells to suspension culture thereof. Pluripotent stem cells are subjected to adherent culture in a liquid medium comprising a PKCβ inhibitor and a TNKS inhibitor and then to suspension culture.