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    ANTIANNEXIN-V MONOCLONAL ANTIBODY, PROCESS FOR PRODUCING THE SAME, AND USE THEROF
    2.
    发明公开
    ANTIANNEXIN-V MONOCLONAL ANTIBODY, PROCESS FOR PRODUCING THE SAME, AND USE THEROF 失效
    MONOKLONALERANTIKÖRPERGEGEN ANTIANNEXIN-V，VERFAHREN ZU SEINER HERSTELLUNG UND SEINER VERWENDUNG

    公开(公告)号：EP0741144A1

    公开(公告)日：1996-11-06

    申请号：EP95936774.9

    申请日：1995-11-10

    申请人： INTERNATIONAL REAGENTS CORPORATION ,  KANEKO, Noboru

    发明人： KANEKO, Noboru ,  MATSUDA, Ryuko ,  KAJITA,Tadahiro International Reagents Corporation ,  OHTA, Yohsuke International Reagents Corporation

    IPC分类号： C07K16/18 ,  C12P21/08 ,  C12N15/06 ,  C12N5/20 ,  G01N33/577

    CPC分类号： C07K16/18 ,  G01N2333/4718

    摘要： Using human annexin-V or human annexin-V plus dog annexin-V as antigen(s), hybridoma cell lines are prepared which are capable of producing anti-annexin-V monoclonal antibodies having a binding specificity to antigenic determinant site on annexin-V as antigenic protein and belonging to immunoglobulin G class. By the hybridoma cell lines are produced the anti-annexin-V monoclonal antibodies, with which a diagnostic agent is provided for diagnosis of myocardial infarction and angina pectoris. There is also provided diagnosis of myocardial infarction and angina pectoris using a first and a second monoclonal antibodies produced by the hybridoma cell lines to quantitate human annexin-V in a sample, in which an antigen-antibody reaction on annexin-V in the sample is caused with the first anti-annexin-V monoclonal antibody to form an annexin-V antigen/anti-annexin-V monoclonal antibody complex, the antigenic site of annexin-V of the formed annexin-V antigen/annexin-V monoclonal antibody complex is allowed to be bound with a labeled anti-annexin-V polyclonal or second monoclonal antibody so as to form a labeled form of said annexin-V antigen/anti-annexin-V monoclonal antibody complex bound with the polyclonal or the second monoclonal antibody, and the labeled form of the complex is quantitatively analyzed.

    摘要翻译： 使用人膜联蛋白-V或人膜联蛋白-V加狗膜联蛋白-V作为抗原，制备能够产生对膜联蛋白-V上的抗原决定簇位点具有结合特异性的抗膜联蛋白-V单克隆抗体的杂交瘤细胞系 作为抗原蛋白，属于免疫球蛋白G类。 通过杂交瘤细胞系产生抗膜联蛋白-V单克隆抗体，通过该单克隆抗体提供诊断剂用于诊断心肌梗死和心绞痛。 还提供使用由杂交瘤细胞系产生的第一和第二单克隆抗体来定量样品中的人膜联蛋白-V的心肌梗塞和心绞痛的诊断，其中样品中膜联蛋白-V上的抗原 - 抗体反应为 由第一抗 - 膜联蛋白-V单克隆抗体引起膜联蛋白-V抗原/抗 - 膜联蛋白-V单克隆抗体复合物，形成的膜联蛋白-V抗原/膜联蛋白-V单克隆抗体复合物的膜联蛋白-V的抗原位点是 允许与标记的抗膜联蛋白-V多克隆或第二单克隆抗体结合，以形成与多克隆或第二单克隆抗体结合的所述膜联蛋白-V抗原/抗 - 膜联蛋白-V单克隆抗体复合物的标记形式，以及 对复合物的标记形式进行定量分析。