公开(公告)号：EP3660157A9

公开(公告)日：2020-07-29

申请号：EP19762287.1

申请日：2019-05-02

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  KIM, Kyung-Jin ,  AHN, Jung Ho ,  SEO, Hogyun ,  LEE, Jong An

IPC: C12N15/74 ,  C12N9/04 ,  C12P7/46

Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.

公开(公告)号：EP3660157A1

公开(公告)日：2020-06-03

申请号：EP19762287.1

申请日：2019-05-02

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  KIM, Kyung-Jin ,  AHN, Jung Ho ,  SEO, Hogyun ,  LEE, Jong An

IPC: C12N15/74 ,  C12N9/04 ,  C12P7/46

Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.