公开(公告)号：EP3961194A1

公开(公告)日：2022-03-02

申请号：EP21192162.2

申请日：2021-08-19

Applicant: Korea Advanced Institute of Science and Technology

Inventor： CHANG, Jae-Byum ,  YOON, Young-Gyu ,  SEO, Junyoung ,  SIM, Yeonbo ,  KIM, Jee Won

IPC: G01N21/64

Abstract: The present disclosure provides a method and apparatus for biomolecular multiplexed imaging through the iterative unmixing of fluorophores. According to the first embodiments of the present disclosure, although signals of two fluorophores are detected in the first fluorescent detection spectral range, the signals of the two images obtained from two detection spectral ranges can be unmixed through the iterative minimization of mutual information. Furthermore, the present disclosure provides a multi-color unmixing method and apparatus through the iterative minimization of mutual information. In the second embodiments of the present disclosure, a plurality of images of a plurality of fluorophores marking different biomolecules, respectively, for example, N fluorophores are obtained. Images, each containing the signals of single fluorophore can be obatined from the obtained images while minimizing the mutual information shared by images of each of pairs each consisting of two of the obtained images.

公开(公告)号：EP4024329A1

公开(公告)日：2022-07-06

申请号：EP22150031.7

申请日：2022-01-03

Applicant: Korea Advanced Institute of Science and Technology

Inventor： CHANG, JaeByum ,  SEO, Junyoung ,  YOON, Young-Gyu ,  KIM, Hyunwoo ,  KIM, Jee Won

IPC: G06T5/50 ,  G06T7/00 ,  G01N21/64

Abstract: This disclosure provides a method and apparatus for multicolor unmixing through iterative mutual information minimization in a simultaneous channel update structure. In this disclosure, a plurality of images may be obtained with respect to a plurality of fluorophores labeled on different biomolecules, respectively. Images of the fluorophores may be unmixed from the obtained images, respectively, while simultaneously minimizing mutual information shared between the obtained images based on all permutation combinations of the obtained images. According to the present disclosure, signals having three or more fluorophores whose emission spectra are overlapped in one spectral range can be more accurately unmixed.