公开(公告)号：EP3680343A2

公开(公告)日：2020-07-15

申请号：EP18853447.3

申请日：2018-09-07

Applicant: Korea Advanced Institute of Science and Technology

Inventor： AHN, Jung Hoon ,  BYUN, Hyunjong ,  PARK, Jiyeon

IPC: C12P21/00 ,  C12N9/24 ,  C07K14/415

Abstract: The present invention provides a method for effective extracellular secretion of a target protein, by preparing a fusion protein connecting LARD3 to the target protein and having pI lowered by adjusting the overall charge of target protein, and by using ABC transporter of a bacterial type 1 secretion system (T1SS). The method can allows a protein be produced at a large amount simply and effectively without a separate purification process.