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1.发明公开
公开(公告)号：EP2514816A2
公开(公告)日：2012-10-24
申请号：EP10837893.6
申请日：2010-12-16
发明人： KIM, Hyong Ha , KIM, Woo Jeong , SUH, Jung Keun
CPC分类号： C12Q1/6895 , C12Q2600/13 , C12Q2600/166 , G01N33/5097
摘要： A method of manufacturing a reference material for determining incorporation of a genetically modified (GM) plant into a sample or analyzing a mixing ratio from a tissue-cultured cell line that is obtained by incubating tissues of either a GM plant or a non-GM plant, and a method of determining incorporation of a GM plant into a sample and analyzing a mixing ratio using the reference material are provided. The reference material for determining the incorporation of a genetically modified (GM) plant a sample or analyzing a mixing ratio using the tissue-cultured cell lines that are obtained by incubating tissues of the GM plant and the non-GM plant can be useful in producing a countless number of populations having the same genetic traits via the tissue culture. Thus, when a culture capacity of the reference material is increased to a large volume, it is possible to obtain a large volume of the reference material having uniform qualities with no quality variation between batches. Unlike the conventional reference materials manufactured using grain powder, a reference material with 100% purity can be obtained as either a GM or non-GM reference material by verifying the purity of the tissue-cultured cell line. Accordingly, it is possible to provide the uniform and stable supply of a reference material having uniform compositions.
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公开(公告)号：EP2514816B1
公开(公告)日：2019-06-26
申请号：EP10837893.6
申请日：2010-12-16
发明人： KIM, Hyong Ha , KIM, Woo Jeong , SUH, Jung Keun
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3.发明公开
公开(公告)号：EP2514816A9
公开(公告)日：2013-04-17
申请号：EP10837893.6
申请日：2010-12-16
发明人： KIM, Hyong Ha , KIM, Woo Jeong , SUH, Jung Keun
CPC分类号： C12Q1/6895 , C12Q2600/13 , C12Q2600/166 , G01N33/5097
摘要： A method of manufacturing a reference material for determining incorporation of a genetically modified (GM) plant into a sample or analyzing a mixing ratio from a tissue-cultured cell line that is obtained by incubating tissues of either a GM plant or a non-GM plant, and a method of determining incorporation of a GM plant into a sample and analyzing a mixing ratio using the reference material are provided. The reference material for determining the incorporation of a genetically modified (GM) plant a sample or analyzing a mixing ratio using the tissue-cultured cell lines that are obtained by incubating tissues of the GM plant and the non-GM plant can be useful in producing a countless number of populations having the same genetic traits via the tissue culture. Thus, when a culture capacity of the reference material is increased to a large volume, it is possible to obtain a large volume of the reference material having uniform qualities with no quality variation between batches. Unlike the conventional reference materials manufactured using grain powder, a reference material with 100% purity can be obtained as either a GM or non-GM reference material by verifying the purity of the tissue-cultured cell line. Accordingly, it is possible to provide the uniform and stable supply of a reference material having uniform compositions.
摘要翻译： 一种制造用于确定将基因修饰（GM）植物掺入样品中的参考材料的方法，或者分析来自通过孵育GM植物或非GM植物的组织获得的组织培养细胞系的混合比 以及确定将GM植物并入样品中并使用该参考物质分析混合比的方法。 用于确定将转基因植物和非转基因植物的组织进行培养而获得的组织培养的细胞系用于确定将基因改造的（GM）植物掺入样品或分析混合比例的参考材料可用于生产 通过组织培养具有相同遗传性状的无数个种群。 因此，当参考材料的培养容量增加到大体积时，有可能获得质量均匀的大量参考材料，批次之间没有质量变化。 与使用颗粒粉末制造的常规参考材料不同，通过验证组织培养的细胞系的纯度，可以获得具有100％纯度的参考材料作为GM或非GM参考材料。 因此，可以提供具有均匀组成的参考材料的均匀和稳定的供应。
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