公开(公告)号：EP1880023B1

公开(公告)日：2011-07-27

申请号：EP06757697.5

申请日：2006-05-11

申请人： Korea Research Institute of Standards and Science

发明人： YANG, In-Chul ,  JANG, Sung-Moon ,  SHI, Lian-Hua ,  PARK, Sang-Ryoul

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q2523/125

摘要： The present invention relates to a method for the quantitative analysis of methyl cytosine in a gene. The method comprises the steps of: (1) treating genomic DNA extracted from a cell or tissue sample with bisulfite so as to maintain methyl cytosine intact and to convert unmethylated cytosine into uracil; (2) selectively amplifying a target gene by PCR using the DNA treated in said step (1) as a template in a reaction mixture containing primers specific for the target gene, dNTP and polymerase; (3) removing non-specific amplification products, the dNTP and the primers from the product amplified in said step (2) to purify the target gene; (4) cleaving the target gene purified in said step (3) into a deoxynucleotide monophosphate (dNMP) level; and (5) separating the dNMP-level product cleaved in said step (4) using capillary electrophoresis, to thereby quantify and measure the content of the methyl cytosine in the target gene. Also, the present invention provides a method for classifying or diagnosing aging, cancer, pathogens, pathogenic contamination, tissues and individuals in a sample, the method comprising measuring the content of methyl cytosine in the sample using the quantitative analysis method of methyl cytosine, and comparing the measured content with the database content of methyl cytosine for aging, cancer, pathogens, tissues or individuals. The inventive method for the quantitative analysis of DNA methylation enables the degree of DNA methylation to be quantified in a highspeed, high-sensitivity and automatable platform, which has not yet been achieved by any of the prior methods.

公开(公告)号：EP1820008A1

公开(公告)日：2007-08-22

申请号：EP05820683.0

申请日：2005-11-16

申请人： Korea Research Institute of Standards and Science

发明人： PARK, Sang-Ryoul ,  YANG, In-Chul ,  KIM, Young-Ho

IPC分类号： G01N27/447

CPC分类号： G01N27/44782 ,  B01L9/527 ,  G01N27/44791

摘要： The present invention is related to a method of separation of compounds by electrophoresis in which the compounds such as genes, proteins, etc. may be analyzed very precisely as samples are introduced directly into the separation tubes of the chip at the collection site, and therefore, it is not necessary to have separate fluid paths or individual sample storing apparatus that have been necessary for the conventional electrophoresis; it is easy to make the chip as the structure of the chip becomes extremely simple, and high-density arrangement of the separation tubes is enabled; and further, the compounds such as genes, proteins, etc. may be analyzed very precisely without interference in the storage tubs by using a non-polar solvent as the solvent of the sample storage tub.

公开(公告)号：EP3267179B1

公开(公告)日：2020-05-06

申请号：EP16759178.3

申请日：2016-03-04

申请人： Korea Research Institute Of Standards And Science

发明人： PARK, Sang-Ryoul ,  YOO, Hee-Bong ,  KIM, Juhwang

IPC分类号： G01N21/03 ,  G01N21/25 ,  G01N21/3577 ,  G01N21/85

公开(公告)号：EP3267179A1

公开(公告)日：2018-01-10

申请号：EP16759178.3

申请日：2016-03-04

申请人： Korea Research Institute Of Standards And Science

发明人： PARK, Sang-Ryoul ,  YOO, Hee-Bong ,  KIM, Juhwang

IPC分类号： G01N21/03 ,  G01N21/25

CPC分类号： G01N21/0303 ,  G01N21/255 ,  G01N21/27 ,  G01N21/3577 ,  G01N21/8507 ,  G01N2021/0346

摘要： The present invention relates to a measurement device including a trace sample-use high sensitivity light absorbing cell, the device comprising: a light absorbing cell containing a capillary tube of which both ends are open hollow shaped; a light absorbing cell mounting block on which one end of the light absorbing cell is mounted, and which comprises a light emitting unit, disposed on the upper portion of the light absorbing cell, for irradiating light to one end of the light absorbing cell; and a light receiving block which comprises a light metering immersion unit disposed in such a manner that the other end of the light absorbing cell is immersed in a sample contained therein, and which detects light that is irradiated to one end of the light absorbing cell and emitted to the other end thereof.

摘要翻译： 本发明涉及一种包括痕量样品用高灵敏度光吸收单元的测量装置，该装置包括：含有毛细管的光吸收单元，该毛细管的两端开口为中空形状; 光吸收单元安装块，所述光吸收单元的一端安装在所述光吸收单元安装块上，并且所述光吸收单元安装块包括发光单元，所述发光单元设置在所述光吸收单元的上部，用于将光照射到所述光吸收单元的一端; 以及光接收块，其包括光计量浸入单元，该光计量浸入单元以光吸收池的另一端浸入其中包含的样品的方式设置，并且检测照射到光吸收池的一端的光和 发射到其另一端。

公开(公告)号：EP1880023A1

公开(公告)日：2008-01-23

申请号：EP06757697.5

申请日：2006-05-11

申请人： Korea Research Institute of Standards and Science

发明人： YANG, In-Chul ,  JANG, Sung-Moon ,  SHI, Lian-Hua, Korea Research Inst. of Standards and Science ,  PARK, Sang-Ryoul

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q2523/125

摘要： The present invention relates to a method for the quantitative analysis of methyl cytosine in a gene. The method comprises the steps of: (1) treating genomic DNA extracted from a cell or tissue sample with bisulfite so as to maintain methyl cytosine intact and to convert unmethylated cytosine into uracil; (2) selectively amplifying a target gene by PCR using the DNA treated in said step (1) as a template in a reaction mixture containing primers specific for the target gene, dNTP and polymerase; (3) removing non-specific amplification products, the dNTP and the primers from the product amplified in said step (2) to purify the target gene; (4) cleaving the target gene purified in said step (3) into a deoxynucleotide monophosphate (dNMP) level; and (5) separating the dNMP-level product cleaved in said step (4) using capillary electrophoresis, to thereby quantify and measure the content of the methyl cytosine in the target gene. Also, the present invention provides a method for classifying or diagnosing aging, cancer, pathogens, pathogenic contamination, tissues and individuals in a sample, the method comprising measuring the content of methyl cytosine in the sample using the quantitative analysis method of methyl cytosine, and comparing the measured content with the database content of methyl cytosine for aging, cancer, pathogens, tissues or individuals. The inventive method for the quantitative analysis of DNA methylation enables the degree of DNA methylation to be quantified in a highspeed, high-sensitivity and automatable platform, which has not yet been achieved by any of the prior methods.

摘要翻译： 本发明涉及定量分析基因中的甲基胞嘧啶的方法。 该方法包括以下步骤：（1）用亚硫酸氢盐处理从细胞或组织样品提取的基因组DNA，以保持甲基胞嘧啶完整并将未甲基化的胞嘧啶转化为尿嘧啶; （2）使用所述步骤（1）中处理的DNA作为模板，在含有靶基因特异性引物，dNTP和聚合酶的反应混合物中通过PCR选择性扩增靶基因; （3）从所述步骤（2）中扩增的产物中去除非特异性扩增产物，dNTP和引物以纯化靶基因; （4）将在所述步骤（3）中纯化的靶基因切割成脱氧核苷酸单磷酸（dNMP）水平; 和（5）使用毛细管电泳分离在所述步骤（4）中裂解的dNMP水平的产物，从而定量和测量靶基因中甲基胞嘧啶的含量。 此外，本发明提供了一种用于分类或诊断样品中老化，癌症，病原体，病原体污染，组织和个体的方法，所述方法包括使用甲基胞嘧啶的定量分析方法测量样品中的甲基胞嘧啶的含量，以及 将测量的内容与用于衰老的甲基胞嘧啶的数据库内容进行比较，癌症，病原体，组织或个体。 用于定量分析DNA甲基化的本发明方法使DNA甲基化的程度能够在高速，高灵敏度和可自动化的平台中量化，这尚未通过任何现有方法实现。