公开(公告)号：EP3730606A1

公开(公告)日：2020-10-28

申请号：EP18892944.2

申请日：2018-12-18

申请人： Kyoto University

发明人： TOGUCHIDA, Junya ,  KAWAI, Shunsuke ,  YOSHITOMI, Hiroyuki ,  CANTAS, Alev

IPC分类号： C12N5/077 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50 ,  C12N5/10

摘要： The present invention provides a method for inducing osteogenic differentiation, the method comprising the following steps of: (1) culturing pluripotent stem cells under feeder-free conditions, (2) culturing the cells in a mixed culture medium of an osteogenic induction medium and a pluripotent stem cell medium, the mixed culture medium containing a ROCK inhibitor and a retinoic acid receptor α or β agonist, and (3) culturing the cells in an osteogenic induction medium containing the retinoic acid receptor α or β agonist. The method for inducing osteogenic differentiation according to the present invention is a simple, short-term, highly efficient and highly reproducible one-procedure method for inducing osteogenic differentiation, wherein the method is suitable for bone regeneration therapies, the development of bone metabolic drugs and the development of novel therapies for bone diseases.