公开(公告)号：EP0879892A1

公开(公告)日：1998-11-25

申请号：EP97911489.9

申请日：1997-11-07

申请人： Kyowa Hakko Co., Ltd.

发明人： SAITOH, Chiaki ,  KUMAZAWA, Hideyo ,  AISAKA, Kazuo ,  MIZUKAMI, Toru ,  KATSUMATA, Ryoichi ,  ANDO, Katsuhiko ,  OCHIAI, Keiko ,  OUCHI, Kozo

IPC分类号： C12P33/00 ,  C12N9/04 ,  C12N1/20 ,  A23L1/29

CPC分类号： C12Y101/99001 ,  A23K20/189 ,  A23L5/25 ,  A23L13/48 ,  A23L15/25 ,  A23L17/65 ,  C12N9/0006 ,  C12P33/00

摘要： A process for preparing a practical cholesterol-lowering compound by converting the cholesterol contained in a food or feed to coprostanol having very low intestinal absorption by utilizing an enzymatic action. The content of cholesterol in cholesterol-containing materials such as meat, eggs, milk, fishery products, and cooked and processed foods containing these products, or feed for animals, poultry, fish culture and the like is lowered by treating these materials with three enzymes, i.e., a cholesterol dehydrogenase having an optimal pH value in a neutral pH range and a 4-cholesten-3-one dehydrogenase and a coprostan-3-one dehydrogenase both having an optimal pH value in a weakly acidic range, or cells containing these enzymes to convert cholesterol to coprostanol.

摘要翻译： 通过利用酶作用将食物或饲料中所含的胆固醇转化成具有非常低的肠吸收的coprostanol来制备实用的降胆固醇化合物的方法。 通过用三种酶处理这些物质，降低含有含胆固醇的材料如肉，蛋，牛奶，渔业产品和含有这些产品的熟化和加工食品中的胆固醇或动物，家禽，鱼类培养物等的含量 ，即在中性pH范围内具有最佳pH值的胆固醇脱氢酶和在弱酸性范围内具有最佳pH值的4-胆甾烯-3-酮脱氢酶和副雄甾-3-酮脱氢酶，或含有这些的细胞 将胆固醇转化为coprostanol的酶。