公开(公告)号：EP2845045A4

公开(公告)日：2016-02-24

申请号：EP13784683

申请日：2013-03-13

Applicant: LEICA BIOSYSTEMS IMAGING INC

Inventor： ZOU YUNLU ,  CRANDALL GREG J ,  OLSON ALLEN

IPC: G02B21/00 ,  G01B9/04 ,  G02B21/24 ,  G02B21/36

CPC classification number: G02B21/006 ,  G02B21/245 ,  G02B21/361 ,  G02B21/365

Abstract: Systems and methods for capturing a digital image of a slide using an imaging line sensor and a focusing line sensor. In an embodiment, a beam-splitter is optically coupled to an objective lens and configured to receive one or more images of a portion of a sample through the objective lens. The beam-splitter simultaneously provides a first portion of the one or more images to the focusing sensor and a second portion of the one or more images to the imaging sensor. A processor controls the stage and/or objective lens such that each portion of the one or more images is received by the focusing sensor prior to it being received by the imaging sensor. In this manner, a focus of the objective lens can be controlled using data received from the focusing sensor prior to capturing an image of a portion of the sample using the imaging sensor.