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    METHOD FOR CONTROLLING GALACTOSYLATION OF RECOMBINANT PROTEIN THROUGH OPTIMIZATION OF CULTURE MEDIUM
    1.
    发明公开
    METHOD FOR CONTROLLING GALACTOSYLATION OF RECOMBINANT PROTEIN THROUGH OPTIMIZATION OF CULTURE MEDIUM 审中-公开
    VERFAHREN ZUR STEUERUNG DER GALACTOSYLIERUNG VON REKOMBINANTEM蛋白质优化DES培养基

    公开(公告)号：EP3101120A4

    公开(公告)日：2017-08-09

    申请号：EP15743963

    申请日：2015-01-29

    申请人： LG LIFE SCIENCES LTD

    发明人： JUNG JUN ,  SONG WON SEOK ,  LEE JUN EOK ,  KIM YEON CHUL

    IPC分类号： C12N5/02 ,  C07K16/00 ,  C07K16/18 ,  C12N5/071 ,  C12P21/00

    CPC分类号： C12P21/005 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/14 ,  C07K2317/41 ,  C12N2500/32 ,  C12N2500/60

    摘要： Disclosed are a method for preparing a target recombinant protein with modulated galactosylation or a method for modulating the galactosylation of a target recombinant protein, including increasing the osmolality of a culture solution of animal cells which express a target recombinant protein during the animal cell culture; a method for preparing a target recombinant protein with modulated galactosylation or a method for modulating the galactosylation of a target recombinant protein, including supplementing asparagine to a culture solution of animal cells which express a target recombinant protein during the animal cell culture; a method for preparing a target recombinant protein with modulated galactosylation or a method for modulating the galactosylation of a target recombinant protein, including increasing the osmolality of an animal cell culture which expresses a target recombinant protein and supplementing asparagine thereto during the animal cell culture; and a target recombinant protein with modulated galactosylation, which is prepared by the method.

    摘要翻译： 公开了用于制备具有经调节的半乳糖基化的靶重组蛋白的方法或调节靶重组蛋白的半乳糖基化的方法，包括在动物细胞培养期间增加表达靶重组蛋白的动物细胞的培养物溶液的渗透压浓度; 用于制备具有经调节的半乳糖基化的靶重组蛋白的方法或用于调节靶重组蛋白的半乳糖基化的方法，包括将天冬酰胺补充至在动物细胞培养期间表达靶重组蛋白的动物细胞的培养液中; 用于制备具有经调节的半乳糖基化的靶重组蛋白的方法或用于调节靶重组蛋白的半乳糖基化的方法，包括增加动物细胞培养物的渗透压，所述动物细胞培养物在动物细胞培养期间表达靶重组蛋白并向其补充天冬酰胺; 以及通过该方法制备的具有经调节的半乳糖基化的靶重组蛋白。