Cloning and expressing restriction endonucleases and modification methylases from caryophanon
    3.
    发明公开
    Cloning and expressing restriction endonucleases and modification methylases from caryophanon 失效
    克罗尼昂和表达von Restriktionsendonukleasen und Modifikationsmethylasen von Karyophanon。

    公开(公告)号:EP0605854A2

    公开(公告)日:1994-07-13

    申请号:EP93120847.4

    申请日:1993-12-23

    IPC分类号: C12N15/55 C12N9/22

    CPC分类号: C12N9/12 C12N9/1007

    摘要: The present invention is directed to recombinant hosts which contain and express the Cla I Type-II restriction endonuclease and/or modification methylase genes. The present invention is also directed to vectors or DNA molecules which contain these gene, and to methods of producing the enzymes. One source of these enzyme is Caryophanon latum, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of the invention.

    摘要翻译: 重组宿主细胞 要求保护编码限制性内切核酸酶(I)的Caryophanon基因,其中(I)能够识别式(II)的回文DNA序列并将其切割在T和C残基之间以形成两碱基5'延伸。