公开(公告)号：EP3092303B1

公开(公告)日：2020-08-19

申请号：EP15700549.7

申请日：2015-01-12

申请人： Laboratoires Expanscience

发明人： BREDIF, Stéphanie ,  BAUDOUIN, Caroline ,  MSIKA, Philippe ,  MELONI, Marisa

IPC分类号： C12N5/071 ,  C07K14/78 ,  C07K14/52 ,  C07K14/495 ,  C12N9/04 ,  G01N33/50

公开(公告)号：EP3092303A1

公开(公告)日：2016-11-16

申请号：EP15700549.7

申请日：2015-01-12

申请人： Laboratoires Expanscience

发明人： BREDIF, Stéphanie ,  BAUDOUIN, Caroline ,  MSIKA, Philippe ,  MELONI, Marisa

IPC分类号： C12N5/071 ,  C07K14/78 ,  G01N33/50

摘要： A first subject of the invention is a skin model characterized in that it comprises keratinocytes capable of overexpressing filaggrin. A second subject of the invention is a method for evaluating the
in vitro activity of a formulation or of at least one active agent on the healing of nipple skin and/or on the reduction of nipple skin inflammation, characterized in that said method comprises at least the following steps: a) bringing said active agent or said formulation into contact with a skin model comprising keratinocytes overexpressing filaggrin and also comprising a lesion; b) measuring the level of production of at least one biological marker in the nipple skin model of step a), characterized in that said biological marker can be chosen from lactate dehydrogenase (LDH), TNFα, filaggrin, TGFβ1 and β1 integrin; c) comparing the level of production of at least one biological marker obtained in step b) with a reference level of production; evaluating the efficacy of said active agent or of said formulation according to the comparison of step c).

摘要翻译： 本发明的第一个主题是皮肤模型，其特征在于它包含能够过度表达聚角蛋白聚糖的角质形成细胞。 本发明的第二个主题是评估制剂或至少一种活性剂在乳头皮肤愈合和/或乳头皮肤炎症减轻方面的体外活性的方法，其特征在于所述方法至少包括 以下步骤：a）使所述活性剂或所述制剂与皮肤模型接触，所述皮肤模型包含过度表达聚角蛋白并且还包含损伤的角质形成细胞; b）测量步骤a）的乳头皮肤模型中至少一种生物标志物的产生水平，其特征在于所述生物标志物可选自乳酸脱氢酶（LDH），TNFα，聚角蛋白，TGFβ1和β1整联蛋白; c）将步骤b）中获得的至少一种生物标志物的生产水平与参考生产水平进行比较; 根据步骤c）的比较评估所述活性剂或所述制剂的功效。