公开(公告)号：EP0846169B1

公开(公告)日：2003-03-19

申请号：EP96926042.1

申请日：1996-07-31

申请人： Lek, Tovarna Farmacevtskih in Kemicnih Izdelkov, D.D. ,  Zavod Republike Slovenije Za Transfuzijo Krvi

发明人： CURIN SERBEC, Vladka ,  SCHUURBIERS, Annette ,  MENART, Viktor ,  GADERC POREKAR, Vladimira ,  STALC, Anton ,  ZUNEC, Petra ,  OCVIRK, Janja ,  STABUC, Borut

IPC分类号： C12N15/06 ,  C07K16/24 ,  C07K16/28 ,  C12N5/20 ,  C12P21/08 ,  A61K39/395 ,  G01N33/577 ,  C07K14/525 ,  C07K14/15

CPC分类号： C07K14/7151 ,  A61K38/00 ,  C07K14/525 ,  C07K16/241 ,  C07K16/2866

摘要： The present invention belongs to the field of pharmaceutical industry and relates to novel monoclonal antibodies and fragments thereof which are bound to the available surface of a human recombinant TNF- alpha , TNF- alpha analogues and TNF- alpha soluble receptors p55 and p75. Further, the invention relates to stable hybridoma cell lines, which lines are capable of producing such monoclonal antibodies, to a process for the preparation thereof and to pharmaceutical preparations containing them. The invention also relates to a method for the quantitative determination of human TNF- alpha , free or complexed with soluble TNF- alpha receptors p55 and p75, and to a kit used therefor. By hitherto disclosed monoclonal antibodies and reagents developed therefrom, it has not been possible to simultaneously determine free TNF- alpha and its soluble receptors p55 and p75 as well as complexes thereof. Another object of the invention is also the TNF- alpha analogue TNF- alpha Cys95His107His108Cys148, which in a complex with TNF- alpha soluble receptors, is used as an antigen for immunization of mice.

公开(公告)号：EP0846169A1

公开(公告)日：1998-06-10

申请号：EP96926042.0

申请日：1996-07-31

申请人： Lek, Tovarna Farmacevtskih in Kemicnih Izdelkov, D.D. ,  Zavod Republike Slovenije Za Transfuzijo Krvi

发明人： CURIN SERBEC, Vladka ,  SCHUURBIERS, Annette ,  MENART, Viktor ,  GADERC POREKAR, Vladimira ,  STALC, Anton ,  ZUNEC, Petra ,  OCVIRK, Janja ,  STABUC, Borut

IPC分类号： C07K14 ,  C07K16 ,  C12N15 ,  A61K38

CPC分类号： C07K14/7151 ,  A61K38/00 ,  C07K14/525 ,  C07K16/241 ,  C07K16/2866

摘要： The present invention belongs to the field of pharmaceutical industry and relates to novel monoclonal antibodies and fragments thereof which are bound to the available surface of a human recombinant TNF-α, TNF-α analogues and TNF-α soluble receptors p55 and p75. Further, the invention relates to stable hybridoma cell lines, which lines are capable of producing such monoclonal antibodies, to a process for the preparation thereof and to pharmaceutical preparations containing them. The invention also relates to a method for the quantitative determination of human TNF-α, free or complexed with soluble TNF-α receptors p55 and p75, and to a kit used therefor. By hitherto disclosed monoclonal antibodies and reagents developed therefrom, it has not been possible to simultaneously determine free TNF-α and its soluble receptors p55 and p75 as well as complexes thereof. Another object of the invention is also the TNF-α analogue TNF-α Cys95His107His108Cys148, which in a complex with TNF-α soluble receptors, is used as an antigen for immunization of mice.