公开(公告)号：EP3320115B1

公开(公告)日：2020-09-02

申请号：EP16742101.5

申请日：2016-07-05

申请人： Life Technologies Corporation

发明人： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

IPC分类号： C12Q1/6834 ,  C12Q1/6874

公开(公告)号：EP3320115A1

公开(公告)日：2018-05-16

申请号：EP16742101.5

申请日：2016-07-05

申请人： Life Technologies Corporation

发明人： MENCHEN, Steven M. ,  XIANG, Guobing ,  THWAR, Prasanna Krishnan ,  FREDIANI, Jeffrey ,  WONG, Allen ,  LUI, Alfred ,  LU, Lily

IPC分类号： C12Q1/68

摘要： A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.