公开(公告)号：EP3172565B1

公开(公告)日：2019-04-10

申请号：EP15744286.4

申请日：2015-07-22

申请人： Loughborough University

发明人： EDWARDS, Alexander Daniel ,  REIS, Nuno Miguel Fernandes

IPC分类号： G01N33/552

公开(公告)号：EP3172565A1

公开(公告)日：2017-05-31

申请号：EP15744286.4

申请日：2015-07-22

申请人： Loughborough University

发明人： EDWARDS, Alexander Daniel ,  REIS, Nuno Miguel Fernandes

IPC分类号： G01N33/552

CPC分类号： B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/06 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0825 ,  B01L2300/0861 ,  B01L2300/161 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0457 ,  G01N33/552

摘要： The present invention provides assay devices having a unitary body with an exterior surface, the unitary body being substantially transparent to visible light and formed from a material having a refractive index in the range 1.26 to 1.40, the refractive index being measured at 20° C. with light of wavelength 589 nm, and wherein the unitary body is formed from a hydrophobic material, and at least two capillary bores extending internally along the unitary body, wherein at least a portion of the surface of each capillary bore includes a hydrophilic layer for retaining an assay reagent, and wherein the hydrophilic layer is also substantially transparent to visible light to allow optical interrogation of the capillary bores through the capillary wall. The present invention also provides assay systems including such assay devices, methods of performing an assay using such assay devices and method of method for manufacturing such assay devices.

摘要翻译： 本发明提供了具有具有外表面的整体主体的测定装置，所述整体主体对可见光基本上是透明的并且由具有在1.26至1.40范围内的折射率的材料形成，所述折射指数在20℃下用 波长为589nm的光，并且其中整体由疏水性材料形成，并且至少两个毛细孔沿着整体本体内部延伸，其中每个毛细孔的至少一部分表面包括亲水层，用于保持 测定试剂，并且其中亲水层对可见光也基本上是透明的，以允许通过毛细管壁对毛细孔进行光学询问。 本发明还提供了包括这种测定装置的测定系统，使用这种测定装置进行测定的方法以及用于制造这种测定装置的方法。