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1.发明授权METHODS AND DEVICE FOR IN VITRO DETECTION AND CHARACTERIZATION OF PSYCHOACTIVES USING ANALYSIS OF REPETITIVE ELECTRICAL ACTIVITY IN A NEURONAL SAMPLE 有权方法和装置体外检测和标签PSYCHO采用电重复性活动的解读IN A神经探针活性物质
公开(公告)号：EP1192461B1
公开(公告)日：2007-04-18
申请号：EP00944867.1
申请日：2000-06-21
IPC分类号： G01N33/483 , G01N33/487 , C12M1/34
CPC分类号： G01N33/48714 , G01N33/48707 , G01N33/5088 , G01N33/94
摘要： This relates to methods for the detection of psychoactive compounds in an in vitro neuronal tissue sample by detecting oscillations of extracellular voltage desirably before and after the introduction of a candidate sample onto an in vitro neuronal tissue sample and for devices useful in practicing the methods. Analysis of the extracellular voltage parameters leads to indication of the presence of psychoactive material in the candidate sample and information as to its pharmacological activity and/or composition. Further, it relates to a process of initiating and maintaining the presence of repetitive neuronal activity within the in vitro sample. Additionally, this includes a method for the stimulation of or initiation of repetitive neuronal activity, e.g., EEG, in an in vitro nueronal tissue sample by introducing a stimulating composition comprising compounds that facilitate or mimic the actions of acetylcholine, serotonin, or catecholamines, such as carbachol, or by subjecting the in vitro neuronal sample to electrical stimulation parameters.
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2.发明授权METHODS AND DEVICE FOR IN VITRO DETECTION AND CHARACTERIZATION OF PSYCHOACTIVES USING ANALYSIS OF REPETITIVE ELECTRICAL ACTIVITY IN A NEURONAL SAMPLE 有权线法和体外检测与表征PSYCHO活性物质的设备通过分析重复电生理学活动IN A神经探针
公开(公告)号：EP1590665B1
公开(公告)日：2006-06-07
申请号：EP04705527.2
申请日：2004-01-27
发明人： LYNCH, Gary , SHIMONO, Ken , TAKETANI, Makoto , SUGIHARA, Hirokazu , COLGIN, Laura, L. , JIA, Yousheng
IPC分类号： G01N33/483 , G01N33/487 , G01N33/50
CPC分类号： G01N33/94 , G01N33/48707 , G01N33/48714 , G01N33/5088
摘要： This invention relates to methods for the detection and characterization of psychoactives in neuronal tissue by comparing parameters measured for a set of baseline oscillations with those same parameters measured for a set of resulting oscillations. The baseline oscillations are induced or stimulated by such agents as chemical compounds that effect cholinergic interactions, co-deposited neuronal tissue, or electrical stimulations. The resulting set of oscillations is obtained after exposing the neuronal tissue to a sample composition that may or may not contain a psychoactive. By comparing the parameters for the baseline and resulting oscillations, the psychoactive in the sample composition may be detected and characterized as belonging to a specific psychoactive compound class and in some instances, may be distinguished from other members of the psychoactive compound class.
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3.发明公开METHODS AND DEVICE FOR IN VITRO DETECTION AND CHARACTERIZATION OF PSYCHOACTIVES USING ANALYSIS OF REPETITIVE ELECTRICAL ACTIVITY IN A NEURONAL SAMPLE 有权线法和体外检测与表征PSYCHO活性物质的设备通过分析重复电生理学活动IN A神经探针
公开(公告)号：EP1590665A2
公开(公告)日：2005-11-02
申请号：EP04705527.2
申请日：2004-01-27
发明人： LYNCH, Gary , SHIMONO, Ken , TAKETANI, Makoto , SUGIHARA, Hirokazu , COLGIN, Laura, L. , JIA, Yousheng
IPC分类号： G01N33/483 , G01N33/487 , G01N33/50
CPC分类号： G01N33/94 , G01N33/48707 , G01N33/48714 , G01N33/5088
摘要： This invention relates to methods for the detection and characterization of psychoactives in neuronal tissue by comparing parameters measured for a set of baseline oscillations with those same parameters measured for a set of resulting oscillations. The baseline oscillations are induced or stimulated by such agents as chemical compounds that effect cholinergic interactions, co-deposited neuronal tissue, or electrical stimulations. The resulting set of oscillations is obtained after exposing the neuronal tissue to a sample composition that may or may not contain a psychoactive. By comparing the parameters for the baseline and resulting oscillations, the psychoactive in the sample composition may be detected and characterized as belonging to a specific psychoactive compound class and in some instances, may be distinguished from other members of the psychoactive compound class.
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公开(公告)号：EP1040345B1
公开(公告)日：2006-03-22
申请号：EP98966077.4
申请日：1998-12-22
发明人： SUGIHARA, Hirokazu , OKA, Hiroaki, Hirakata Garden Hills 913 , SHIMONO, Ken , OGAWA, Ryuta , TAKETANI, Makoto
IPC分类号： G01N27/403 , G01N33/487 , C12M1/34
CPC分类号： G01N33/4836
摘要： This invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.
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公开(公告)号：EP1040345A1
公开(公告)日：2000-10-04
申请号：EP98966077.4
申请日：1998-12-22
发明人： SUGIHARA, Hirokazu , OKA, Hiroaki,Hirakata Garden Hills 913 , SHIMONO, Ken , OGAWA, Ryuta , TAKETANI, Makoto
IPC分类号： G01N27/403 , G01N33/487 , C12M1/34
CPC分类号： G01N33/4836
摘要： This invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.
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公开(公告)号：EP1673467A2
公开(公告)日：2006-06-28
申请号：EP04796049.7
申请日：2004-10-15
IPC分类号： C12Q1/00
CPC分类号： A61K33/00 , A61K33/40 , G01N33/48707 , G01N33/5438 , G01N2500/00
摘要： The invention relates to systems and methods for assessing neuronal degeneration in vitro. A multielectrode probe is used to culture and measure synaptic transmission of a neuronal sample in the presence of various compounds and culturing conditions capable of inducing or protecting against neuronal damage.
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7.发明公开METHODS AND DEVICE FOR i IN VITRO /i DETECTION AND CHARACTERIZATION OF PSYCHOACTIVES USING ANALYSIS OF REPETITIVE ELECTRICAL ACTIVITY IN A NEURONAL SAMPLE 有权方法和装置体外检测和标签PSYCHO采用电重复性活动的解读IN A神经探针活性物质
公开(公告)号：EP1192461A2
公开(公告)日：2002-04-03
申请号：EP00944867.1
申请日：2000-06-21
IPC分类号： G01N33/483 , G01N33/487 , C12M1/34
CPC分类号： G01N33/48714 , G01N33/48707 , G01N33/5088 , G01N33/94
摘要： This relates to methods for the detection of psychoactive compounds in an in vitro neuronal tissue sample by detecting oscillations of extracellular voltage desirably before and after the introduction of a candidate sample onto an in vitro neuronal tissue sample and for devices useful in practicing the methods. Analysis of the extracellular voltage parameters leads to indication of the presence of psychoactive material in the candidate sample and information as to its pharmacological activity and/or composition. Further, it relates to a process of initiating and maintaining the presence of repetitive neuronal activity within the in vitro sample. Additionally, this includes a method for the stimulation of or initiation of repetitive neuronal activity, e.g., EEG, in an in vitro nueronal tissue sample by introducing a stimulating composition comprising compounds that facilitate or mimic the actions of acetylcholine, serotonin, or catecholamines, such as carbachol, or by subjecting the in vitro neuronal sample to electrical stimulation parameters.
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