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    METHOD OF INTEGRATING GENE INTO CHROMOSOME OF $i(LACTOBACILLUS DELBRUECKII) SPECIES AND PRODUCT OF GENE INTEGRATION
    1.
    发明公开
    METHOD OF INTEGRATING GENE INTO CHROMOSOME OF $i(LACTOBACILLUS DELBRUECKII) SPECIES AND PRODUCT OF GENE INTEGRATION 失效
    METHOD FOR整合基因到染色体甲-i（LACTOKACILLUS德氏）种及产品，遗传融合。

    公开(公告)号：EP0603416A1

    公开(公告)日：1994-06-29

    申请号：EP93914972.0

    申请日：1993-07-08

    申请人： MEIJI MILK PRODUCTS COMPANY LIMITED

    发明人： SASAKI, Takashi ,  SASAKI, Yasuko ,  ITO, Yoshiyuki ,  OTSU, Kumi

    IPC分类号： C12N15/90 ,  C12N1/21

    CPC分类号： C12N9/0006 ,  C12N15/746 ,  C12N15/902

    摘要： A product of gene integration prepared by integrating an arbitrary gene into a chromosomal DNA of a bacillus of Lactobacillus delbrueckii species through a method which comprises constructing an integration plasmid by combining pAMβ1 plasmid with a target gene, subjecting the obtained plasmid to conjugation transfer and homologous recombination to thereby integrate only the target gene into the chromosomal DNA, and eliminating the DNA sequence of the pAMβ1 as a vector. The product can be widely applied to the production of food, drug and feed.

    摘要翻译： 通过INTEGRA婷准备任意的基因导入通过方法德氏乳杆菌属物种的芽孢杆菌的染色体DNA的基因整合的一种产品，它通过组合PAM的β1个质粒与靶基因，对所得质粒接合转移，并且包括整合质粒的构建 同源重组，从而整合只有目标基因到染色体DNA中，和消除的βPAM 1作为载体的DNA序列。 该产品可广泛应用于生产食品，药品和饲料。