公开(公告)号：EP0327312A2

公开(公告)日：1989-08-09

申请号：EP89300928.2

申请日：1989-01-31

申请人： MICROGENICS CORPORATION (a Delaware corporation)

发明人： Khanna, Pyare L. ,  Friedman, Stephen B. ,  Dworschak, Robert T.

IPC分类号： G01N33/58 ,  G01N33/535 ,  G01N33/94 ,  G01N33/78

CPC分类号： G01N33/581 ,  G01N33/542

摘要： A β-galactosidase complementation immunoassay method for determining the concentration of an analyte in a sample is provided. The method is based on com­bining a preformed enzyme donor- (ED-) analyte conju­gate/anti-analyte antibody complex with analyte present in the sample. The method comprises reacting a β-galactosidase ED-analyte conjugate/anti-analyte anti­body complex, a β-galactosidase enzyme acceptor (EA), enzyme substrate and sample and determining the rate of enzyme-catalyzed reaction as indicative of the amount of analyte present in the sample. Usually, the EA is added to the reaction mixture after a time for the anti-analyte antibody of the complex to react with analyte present in the sample.
In a particular embodiment, the method is used to determine the amount of digoxin in a serum sample. In another embodiment the method is used to determine the amount of T3 in a sample. Kits facilitating the method are also provided.

摘要翻译： 提供了用于确定样品中分析物浓度的β-半乳糖苷酶互补免疫测定方法。 该方法基于将预先形成的酶供体 - （ED-）分析物缀合物/抗分析物抗体复合物与样品中存在的分析物组合。 该方法包括使β-半乳糖苷酶ED-分析物缀合物/抗分析物抗体复合物，β-半乳糖苷酶酶接受体（EA），酶底物和样品反应，并确定酶催化反应的速率作为存在的分析物的量的指示 在样本中。 通常，在复合物的抗分析物抗体与样品中存在的分析物反应一段时间之后，将EA添加到反应混合物中。 在一个具体的实施方案中，该方法用于确定血清样品中地高辛的量。 在另一个实施方案中，该方法用于确定样品中T3的量。 还提供了便于该方法的试剂盒。

公开(公告)号：EP0327312B1

公开(公告)日：1994-04-06

申请号：EP89300928.2

申请日：1989-01-31

申请人： MICROGENICS CORPORATION (a Delaware corporation)

发明人： Khanna, Pyare L. ,  Friedman, Stephen B. ,  Dworschak, Robert T.

IPC分类号： G01N33/58 ,  G01N33/535 ,  G01N33/94 ,  G01N33/78

CPC分类号： G01N33/581 ,  G01N33/542

公开(公告)号：EP0327312A3

公开(公告)日：1990-08-22

申请号：EP89300928.2

申请日：1989-01-31

申请人： MICROGENICS CORPORATION (a Delaware corporation)

发明人： Khanna, Pyare L. ,  Friedman, Stephen B. ,  Dworschak, Robert T.

IPC分类号： G01N33/58 ,  G01N33/535 ,  G01N33/94 ,  G01N33/78

CPC分类号： G01N33/581 ,  G01N33/542

摘要： A β-galactosidase complementation immunoassay method for determining the concentration of an analyte in a sample is provided. The method is based on com­bining a preformed enzyme donor- (ED-) analyte conju­gate/anti-analyte antibody complex with analyte present in the sample. The method comprises reacting a β-galactosidase ED-analyte conjugate/anti-analyte anti­body complex, a β-galactosidase enzyme acceptor (EA), enzyme substrate and sample and determining the rate of enzyme-catalyzed reaction as indicative of the amount of analyte present in the sample. Usually, the EA is added to the reaction mixture after a time for the anti-analyte antibody of the complex to react with analyte present in the sample. In a particular embodiment, the method is used to determine the amount of digoxin in a serum sample. In another embodiment the method is used to determine the amount of T3 in a sample. Kits facilitating the method are also provided.