公开(公告)号：EP1298217A4

公开(公告)日：2004-09-08

申请号：EP01949913

申请日：2001-07-04

申请人： MITSUBISHI PHARMA CORP

发明人： SUGA KENICHI ,  OMASA TAKESHI ,  KISHIMOTO MICHIMASA ,  KATAKURA YOSHIO ,  OHDA TOYOO ,  MIKI HIDEO ,  KOBAYASHI KAORU

IPC分类号： C12P21/00

CPC分类号： C12P21/005

摘要： A means of controlling sugar chain-modification and sugar chain structure in the production of a glycoprotein with the use of gene recombination techniques. A process for producing a glycoprotein by culturing a gene recombinant host in a medium characterized in that the relative sugar consumption speed is employed as an indication and changed to thereby modify the sugar chain structure attached to the protein.

公开(公告)号：EP1004673A4

公开(公告)日：2003-01-15

申请号：EP98911202

申请日：1998-04-03

申请人： MITSUBISHI PHARMA CORP

发明人： OHDA TOYOO ,  OHYA TOMOSHI ,  KUWAE SHINOBU ,  OHYAMA MASAO ,  KOBAYASHI KAORU ,  UEMURA YAHIRO

IPC分类号： C12N1/16 ,  C12P21/02 ,  C12P21/00

CPC分类号： C12N1/16 ,  C12P21/02

摘要： A process for producing foreign proteins characterized by culturing foreign protein-producing hosts constructed by gene manipulation in media containing fatty acids or salts thereof and surfactants and harvesting the foreign proteins from the cultures. Thus the yields of the foreign proteins produced by the hosts can be elevated. Moreover, the degradation of the foreign proteins by enzymes originating in the hosts can be thus inhibited, which enables mass production of the foreign proteins.