公开(公告)号：EP0683232B1

公开(公告)日：2001-02-14

申请号：EP94931697.0

申请日：1994-11-09

申请人： MITSUI CHEMICALS, INC.

发明人： YUKIMUNE, Yukihito ,  HARA, Yasuhiro Mitsui Petrochemical Industries Ltd ,  HIGASHI, Yosuke ,  OHNISHI, Naoto Mitsui Petrochemical Industries Ltd ,  TABATA, Homare Mitsui Petrochemical Industries Ltd ,  SUGA, Chuzo Mitsui Petrochemical Industries, Ltd. ,  MATSUBARA, Kouichi Mitsui Petrochemical Ind. Ltd.

IPC分类号： C12P17/02 ,  C12N5/04

CPC分类号： C12N5/04 ,  C12N5/0025 ,  C12P17/02

摘要： A process for producing a taxane diterpene which comprises culturing the tissue or cell of a plant that produces a taxane diterpene in the presence of at least one member selected from the group consisting of jasmonic acids, heavy metal compounds, heavy metal complex ions, heavy metal ions, amines and ethylene-resisting agent; another process for producing a taxane diterpene which comprises culturing the tissue or cell by controlling the condition from the outset such that the oxygen concentration in the gas phase within the culture vessel is lower than the atmospheric oxygen concentration or that the dissolved oxygen concentration in the flowable medium in contact with the tissue or cell is lower than the saturation dissolved oxygen concentration at that temperature; still another process for producing a taxane diterpene which comprises culturing the tissue or cell in a culture tank by flowing into the tank an oxygenic gas containing 0.03-10 % of carbon dioxide; and a method of harvesting a cultured cell capable of producinga taxane diterpene in a high yield which comprises distributing the cells among a number of layers on the basis of a difference in specific gravity, culturing the cells contained in at least one of the layers, and selecting the target cell from among the cells thus cultured. The invention permits industrial production of taxane diterpenes such as taxol useful as a cancer remedy.