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1.发明公开
公开(公告)号：EP0143849A1
公开(公告)日：1985-06-12
申请号：EP84901004.6
申请日：1984-03-01
CPC分类号： C07H21/00
摘要： A process for the scission of a labelled DNA chemically modified base-selectively at the chemically modified position to chemically determined DNA base sequence, which comprises modifying DNA with a chemical reagent, bringing the reaction solution into contact with an adsorbent to adsorb the chemically modified DNA, washing the adsorbent with a washing solution to remove the chemically modifying agent, eluting the adsorbed modified DNA with an eluent, concentrating the eluate, adding a scission reagent thereto, and heating the solution. This process is conducted using an apparatus in which a reactor (1) for retaining the DNA modification reaction solution, a liquid-transport pump (4), an adsorbent-containing apparatus (2), a cross valve (5), and a condenser (3) are mutually connected through pipes.
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公开(公告)号：EP0143849B1
公开(公告)日：1991-08-07
申请号：EP84901004.6
申请日：1984-03-01
CPC分类号： C07H21/00
摘要： A process for the scission of a labelled DNA chemically modified base-selectively at the chemically modified position to chemically determined DNA base sequence, which comprises modifying DNA with a chemical reagent, bringing the reaction solution into contact with an adsorbent to adsorb the chemically modified DNA, washing the adsorbent with a washing solution to remove the chemically modifying agent, eluting the adsorbed modified DNA with an eluent, concentrating the eluate, adding a scission reagent thereto, and heating the solution. This process is conducted using an apparatus in which a reactor (1) for retaining the DNA modification reaction solution, a liquid-transport pump (4), an adsorbent-containing apparatus (2), a cross valve (5), and a condenser (3) are mutually connected through pipes.
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