公开(公告)号：EP3021682A1

公开(公告)日：2016-05-25

申请号：EP14737478.9

申请日：2014-06-20

申请人： MJN U.S. Holdings, LLC

发明人： BANAVARA, Dattatreya, ,  ALVEY, John D., ,  PETERS, Joseph Andrew, ,  GONZALEZ, Juan M.,

IPC分类号： A23J1/20 ,  C07K14/79 ,  C07K1/16

摘要： The present disclosure relates to improved expanded bed adsorption processes for isolating proteins from milk sources. In particular embodiments, the present disclosure provides a process for isolating a milk protein, such as lactoferrin, from a milk source comprising establishing an expanded bed adsorption column comprising a particulate matrix, applying a milk source to the matrix, and eluting the lactoferrin from the matrix with an elution buffer comprising about 0.3 to about 2.0 M sodium chloride.

摘要翻译： 本公开涉及用于从乳源分离蛋白质的改进的扩展床吸附方法。 在具体实施方案中，本公开提供了从乳源分离乳蛋白（例如乳铁蛋白）的方法，包括建立包含颗粒基质的膨胀床吸附柱，将乳源施加到基质上，并从其中洗脱乳铁蛋白 基质，洗脱缓冲液包含约0.3至约2.0M氯化钠。