公开(公告)号：EP1156329A2

公开(公告)日：2001-11-21

申请号：EP01303960.7

申请日：2001-04-30

申请人： MOLECULAR DEVICES CORPORATION

发明人： Huang, Wei ,  Sportsman, j. Richard ,  Terpetschung, Ewald A. ,  Hoekstra, Merl F.

IPC分类号： G01N33/53 ,  G01N33/542 ,  C12Q1/48 ,  C12Q1/42

CPC分类号： C07K7/06 ,  B01L3/50853 ,  C07K7/08 ,  C12Q1/485 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/566 ,  G01N33/582 ,  G01N35/028 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/0405 ,  G01N2035/0425 ,  G01N2035/0462 ,  G01N2333/9121 ,  Y10S436/80 ,  Y10S436/805

摘要： An assay method which involves: contacting a sample with a binding partner which binds specifically to a first substrate molecule which may be in the sample but not to a second substrate molecule which may be in the sample; and detecting a response indicative of binding, or of the extent of binding, between the first substrate and the binding partner, characterised in that the binding partner includes a metal ion that is required for binding between the binding partner and the substrate or product and that the response detection is performed without separating the bound first substrate from the unbound first substrate.

摘要翻译： 一种测定方法，其包括：将样品与结合配偶体接触，所述结合配偶体特异性结合可能在样品中的第一底物分子，但不与可能在样品中的第二底物分子结合; 并且检测指示所述第一底物和所述结合配偶体之间的结合或结合程度的响应，其特征在于所述结合配偶体包括在所述结合配偶体与所述底物或产物之间结合所需的金属离子，并且 执行响应检测，而不将结合的第一衬底与未结合的第一衬底分离。

公开(公告)号：EP1156329A3

公开(公告)日：2004-03-03

申请号：EP01303960.7

申请日：2001-04-30

申请人： MOLECULAR DEVICES CORPORATION

发明人： Huang, Wei ,  Sportsman, j. Richard ,  Terpetschung, Ewald A. ,  Hoekstra, Merl F.

IPC分类号： G01N33/53 ,  G01N33/542 ,  C12Q1/48 ,  C12Q1/42

CPC分类号： C07K7/06 ,  B01L3/50853 ,  C07K7/08 ,  C12Q1/485 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/566 ,  G01N33/582 ,  G01N35/028 ,  G01N35/1011 ,  G01N35/1074 ,  G01N2035/00237 ,  G01N2035/0405 ,  G01N2035/0425 ,  G01N2035/0462 ,  G01N2333/9121 ,  Y10S436/80 ,  Y10S436/805

摘要： An assay method which involves: contacting a sample with a binding partner which binds specifically to a first substrate molecule which may be in the sample but not to a second substrate molecule which may be in the sample; and detecting a response indicative of binding, or of the extent of binding, between the first substrate and the binding partner, characterised in that the binding partner includes a metal ion that is required for binding between the binding partner and the substrate or product and that the response detection is performed without separating the bound first substrate from the unbound first substrate.