公开(公告)号：EP1098961B1

公开(公告)日：2008-01-16

申请号：EP99927440.0

申请日：1999-06-11

申请人： MOUNT SINAI SCHOOL OF MEDICINE OF THE CITY UNIVERSITY OF NEW YORK ,  Egorov, Andrej Y., Dr. ,  Muster, Thomas

发明人： EGOROV, Andrej Y., Dr. ,  MUSTER, Thomas ,  GARCIA-SASTRE, Adolfo ,  PALESE, Peter ,  BRANDT, Sabine

IPC分类号： C12N7/01 ,  C12N7/04 ,  C12N15/86 ,  A61K39/145 ,  A61K39/295

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/525 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N15/86 ,  C12N2760/16121 ,  C12N2760/16122 ,  C12N2760/16132 ,  C12N2760/16134 ,  C12N2760/16161 ,  C12N2830/002 ,  Y02A50/412 ,  Y02A50/466

摘要： The present invention relates to genetically engineered attenuated viruses and methods for their production. In particular, the present invention relates to engineering live attenuated viruses which contain a modified NS gene segment. Recombinant DNA techniques can be utilized to engineer site specific mutations into one or more noncoding regions of the viral genome which result in the down-regulation of one or more viral genes. Alternatively, recombinant DNA techniques can be used to engineer a mutation, including but not limited to an insertion, deletion, or substitution of an amino acid residue(s) or an epitope(s) into a coding region of the viral genome so that altered or chimeric viral proteins are expressed by the engineered virus.