公开(公告)号：EP3079003A1

公开(公告)日：2016-10-12

申请号：EP15163243.7

申请日：2015-04-10

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： Huisken, Jan ,  Shah, Gopi

IPC分类号： G02B21/32 ,  G02B21/36

CPC分类号： G02B21/367 ,  F16C32/0406 ,  G01N21/01 ,  G02B21/32

摘要： Method for imaging regions of a sample using a light source and an optical detection means and at least one device for moving the sample in three dimensions, comprising the following method steps:
a) introducing at least one magnetic element into the sample,
b) applying a magnetic field by means of the at least one device for moving the sample in three dimensions, the magnetic field interacting with the at least one magnetic element introduced into the sample,
c) arranging the region of the sample in a radiation region of the light source and in a detection region of the detection means,
d) emitting first light beams from the light source onto the sample,
e) generating second light beams by means of the sample,
f) recording an image of a region of the sample by capturing a proportion, incident on the detection means from the sample, of the second light beams,
g) moving the at least one magnetic element and the sample containing this at least one magnetic element by varying the magnetic field,
h) repeating steps d) to g) until a predeterminable number of images have been recorded.

摘要翻译： 用于使用光源和光学检测装置对样品进行成像的方法和用于三维移动样品的至少一个装置，包括以下方法步骤：a）将至少一个磁性元件引入样品中，b）施加 通过所述至少一个用于在三维上移动所述样本的装置的磁场，与所述至少一个引入到所述样品中的所述至少一个磁性元件相互作用的磁场; c）将所述样品的区域布置在所述光的辐射区域中 源和在检测装置的检测区域中，d）将来自光源的第一光束发射到样品上，e）通过样品产生第二光束，f）通过捕获来记录样品区域的图像 从样品入射到检测装置上的第二光束的比例，g）通过改变磁性元件移动至少一个磁性元件和包含该至少一个磁性元件的样品 字段，h）重复步骤d）至g）直到已经记录了可预定数量的图像。