公开(公告)号：EP1417485A2

公开(公告)日：2004-05-12

申请号：EP02754011.1

申请日：2002-08-07

申请人： McGILL UNIVERSITY ,  Chronogen

发明人： HEKIMI, Siegfried ,  HIHI, Abdelmadjid ,  LEVAVASSEUR, Françoise ,  SHOUBRIDGE, Eric ,  GAO, Yuan ,  PAQUET, Michel ,  BENARD, Claire

IPC分类号： G01N33/50

CPC分类号： A01K67/0276 ,  A01K67/0336 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2227/703 ,  A01K2267/03 ,  A61K49/0008 ,  C12N9/0073 ,  C12N9/1007 ,  C12N15/8509 ,  C12N2800/30 ,  G01N33/5085 ,  G01N33/5088 ,  G01N2500/00

摘要： The present invention relates to a method of screening for a compound allowing survival of clk1 homozygous mutant embryos; a method of screening for a compound suitable for rescue of mutant phenotype of mclk1 homozygous cell line; a method of screening for a compound suitable for partial or complete functional replacement of endogenous ubiquinone; a method for screening a compound capable of inhibiting activity of clk-1 and/or other processes required to make ubiquinone from demethoxyubiquinone; a non-ubiquinone-producer mouse; a DNA construct, which comprises an alteration of mclk1; a non-ubiquinone-producer ES cell line; a coq-3 mutant subject non-ubiquinone producer; a method of screening for a compound suitable for complete or partial functional ubiquinone or demethoxyubiquinone replacement; a method for reducing and/or increasing ubiquinone level in a multicellular subject; a method of screening for a genetic suppressor of clk-1; and a method of screening for a genetic suppressor of coq-3.