公开(公告)号：EP2970949B1

公开(公告)日：2018-05-30

申请号：EP14711216.3

申请日：2014-03-14

申请人： MedImmune Limited

发明人： DIECI, Giorgio ,  MARELLI, Marcello ,  GRABSTEIN, Kenneth H.

IPC分类号： C12N15/09 ,  C12N15/11

CPC分类号： C12N15/85 ,  C12N15/67 ,  C12N2800/107 ,  C12P21/00

摘要： There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system.

公开(公告)号：EP2970949A2

公开(公告)日：2016-01-20

申请号：EP14711216.3

申请日：2014-03-14

申请人： MedImmune Limited

发明人： DIECI, Giorgio ,  MARELLI, Marcello ,  GRABSTEIN, Kenneth H.

IPC分类号： C12N15/09 ,  C12N15/11

CPC分类号： C12N15/85 ,  C12N15/67 ,  C12N2800/107 ,  C12P21/00

摘要： There is provided inter alia a DNA construct which comprises a tRNApyl coding sequence and a RNA polymerase III promoter sequence which is capable of acting to express functional tRNApyl sufficiently to support nonsense suppression in a eukaryotic expression system.

摘要翻译： 特别提供了一种DNA构建体，其包含tRNA基因编码序列和RNA聚合酶III启动子序列，其能够充分表达功能性tRNA基，以支持真核表达系统中的无义抑制。