公开(公告)号：EP1097988B1

公开(公告)日：2008-12-31

申请号：EP01200292.9

申请日：1993-05-27

申请人： Merck & Co., Inc.

发明人： Provost, Phillip J. ,  Krah, David L. ,  Friedman, Paul A.

IPC分类号： C12N5/00 ,  C12N5/08

CPC分类号： C12N5/0018 ,  A61K39/12 ,  A61K39/25 ,  A61K2039/5254 ,  C12N2500/36 ,  C12N2710/16734 ,  Y02A50/466

摘要： A process for culturing cells in monolayer which comprises : a. seeding a culture vessel with a cell stock in a minimal or rich medium and allowing the cells to plant for about 24-72 hours; b. removing the medium from the culture of step a. and replacing with fresh, rich medium, supplemented with an opitimized concentration of lipids and optionally also including a serum supplement; c. growing the culture of cells in rich medium supplemented with lipid; d . optionally replacing the medium of Step c. with fresh medium containing no lipid supplement, after about 24-72 hours growth.

公开(公告)号：EP0573107A3

公开(公告)日：1995-04-26

申请号：EP93201521.7

申请日：1993-05-27

申请人： MERCK & CO. INC.

发明人： Provost, Phillip J. ,  Krah, David L. ,  Friedman, Paul A.

IPC分类号： A61K39/25

CPC分类号： C12N5/0018 ,  A61K39/12 ,  A61K39/25 ,  A61K2039/5254 ,  C12N2500/36 ,  C12N2710/16734 ,  Y02A50/466

摘要： A live, attenuated varicella zoster virus vaccine is produced with enhanced yield of VZV. The new process makes mass production of a live VZV vaccine more practical. In addition, optimized monolayer cell culture conditions provide a process for maximizing monolayer cell density which is useful for enhancing viral vaccine production. According to this process, cell densities approaching 500,000 cells/cm² are routinely achieved in conventional culture vessels.

公开(公告)号：EP0573107B1

公开(公告)日：2001-10-04

申请号：EP93201521.7

申请日：1993-05-27

申请人： Merck & Co., Inc.

发明人： Provost, Phillip J. ,  Krah, David L. ,  Friedman, Paul A.

IPC分类号： A61K39/25

CPC分类号： C12N5/0018 ,  A61K39/12 ,  A61K39/25 ,  A61K2039/5254 ,  C12N2500/36 ,  C12N2710/16734 ,  Y02A50/466

摘要： A process for culturing cells in monolayer which comprises : a. seeding a culture vessel with a cell stock in a minimal or rich medium and allowing the cells to plant for about 24-72 hours; b. removing the medium from the culture of step a. and replacing with fresh, rich medium, supplemented with an opitimized concentration of lipids and optionally also including a serum supplement; c. growing the culture of cells in rich medium supplemented with lipid; d . optionally replacing the medium of Step c. with fresh medium containing no lipid supplement, after about 24-72 hours growth.

公开(公告)号：EP1097988A1

公开(公告)日：2001-05-09

申请号：EP01200292.9

申请日：1993-05-27

申请人： Merck & Co., Inc.

发明人： Provost, Phillip J. ,  Krah, David L. ,  Friedman, Paul A.

IPC分类号： C12N5/00 ,  C12N5/08

CPC分类号： C12N5/0018 ,  A61K39/12 ,  A61K39/25 ,  A61K2039/5254 ,  C12N2500/36 ,  C12N2710/16734 ,  Y02A50/466

摘要： A process for culturing cells in monolayer which comprises :

a. seeding a culture vessel with a cell stock in a minimal or rich medium and allowing the cells to plant for about 24-72 hours;
b. removing the medium from the culture of step a. and replacing with fresh, rich medium, supplemented with an opitimized concentration of lipids and optionally also including a serum supplement;
c. growing the culture of cells in rich medium supplemented with lipid;
d . optionally replacing the medium of Step c. with fresh medium containing no lipid supplement, after about 24-72 hours growth.

摘要翻译： 一种单层培养细胞的方法，包括：a。 将细胞储存在最小或富含培养基中的培养容器接种，并允许细胞植物约24-72小时; 湾 从步骤a的培养物中去除培养基。 并用新鲜的富含培养基替代，补充有优化的脂质浓度，并任选地还包括血清补充剂; C。 在富含脂质的培养基中培养细胞培养; d。 可选地替换步骤c的介质。 在不含脂质补充剂的新鲜培养基中培养约24-72小时。

公开(公告)号：EP0573107A2

公开(公告)日：1993-12-08

申请号：EP93201521.7

申请日：1993-05-27

申请人： MERCK & CO. INC.

发明人： Provost, Phillip J. ,  Krah, David L. ,  Friedman, Paul A.

IPC分类号： A61K39/25

CPC分类号： C12N5/0018 ,  A61K39/12 ,  A61K39/25 ,  A61K2039/5254 ,  C12N2500/36 ,  C12N2710/16734 ,  Y02A50/466

摘要： A live, attenuated varicella zoster virus vaccine is produced with enhanced yield of VZV. The new process makes mass production of a live VZV vaccine more practical. In addition, optimized monolayer cell culture conditions provide a process for maximizing monolayer cell density which is useful for enhancing viral vaccine production. According to this process, cell densities approaching 500,000 cells/cm² are routinely achieved in conventional culture vessels.

摘要翻译： 生产具有增强的VZV产量的活的，减毒的水痘带状疱疹病毒疫苗。 新工艺使大量生产活VZV疫苗更加实用。 此外，优化的单层细胞培养条件提供了最大化单层细胞密度的方法，其可用于增强病毒疫苗生产。 根据该方法，在常规培养容器中常规地实现接近50万个细胞/ cm 2的细胞密度。