公开(公告)号：EP4349976A1

公开(公告)日：2024-04-10

申请号：EP22811031.8

申请日：2022-03-31

申请人： Mican Technologies Inc. ,  Japan as Represented by The Director-General of National Institute of Infectious Diseases ,  OSAKA UNIVERSITY

发明人： SUZUKI, Ryosuke ,  MATSUDA, Mami ,  MURAMATSU, Masamichi ,  SAITO, Kyoko ,  FUKASAWA, Masayoshi ,  HANADA, Kentaro ,  YAMANAKA, Atsushi ,  MIYAZAKI, Kazuo ,  YAMADA, Misuzu ,  SHIMIZU, Jun

IPC分类号： C12N7/01 ,  C12N5/10 ,  C12N15/33 ,  C12Q1/02 ,  G01N33/53

摘要： The present invention aims to provide a method for testing the function of antibodies that uses safe antigens and gives results more quickly. The present invention relates to a method for determining antibody-dependent enhancement ability of antibodies, including contacting, in the presence of a test antibody, Fcγ receptor-expressing cell with single round infectious virus particles containing a gene with a region encoding a labeled protein and a region encoding non-structural (NS) proteins 1 to 5 of the yellow fever virus genome, a capsid protein of a virus, and an outer shell protein (Envelope) of a virus, wherein when the measured label is greater than that of a negative control cell, the test antibody is determined to have an antibody-dependent enhancement ability, and the like.