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    DNA RANDOM ACCESS STORAGE SYSTEM VIA LIGATION
    5.
    发明公开
    DNA RANDOM ACCESS STORAGE SYSTEM VIA LIGATION 审中-公开

    公开(公告)号：EP3513346A1

    公开(公告)日：2019-07-24

    申请号：EP17800658.1

    申请日：2017-10-30

    申请人： Microsoft Technology Licensing, LLC

    发明人： STRAUSS, Karin ,  CHEN, Yuan-Jyue

    IPC分类号： G06F19/28 ,  C12Q1/6855 ,  C12Q1/6869

    摘要： Techniques for random access of particular DNA strands from a mixture of DNA strands are described. DNA strands that encode pieces of the same digital file are labeled with the same identification sequence. The identification sequence is used to selectively separate DNA strands that contain portions of the same digital file from other DNA strands. A DNA staple positions DNA strands with the identification sequence adjacent to sequencing adaptors. DNA ligase joins the molecules to create a longer molecule with the region encoding the digital file flanked by sequencing adaptors. DNA strands that include sequencing adaptors are sequenced and the sequence data is available for further analysis. DNA strands without the identification sequence are not joined to sequencing adaptors, and thus, are not sequenced. As a result, the sequencing data produced by the DNA sequencer comes from those DNA strands that included the identification sequence.

    HOMOPOLYMER PRIMERS FOR AMPLIFICATION OF POLYNUCLEOTIDES CREATED BY ENZYMATIC SYNTHESIS
    7.
    发明公开
    HOMOPOLYMER PRIMERS FOR AMPLIFICATION OF POLYNUCLEOTIDES CREATED BY ENZYMATIC SYNTHESIS 审中-实审

    公开(公告)号：EP4397772A2

    公开(公告)日：2024-07-10

    申请号：EP24173096.9

    申请日：2021-03-16

    申请人： Microsoft Technology Licensing, LLC

    发明人： CHEN, Yuan-Jyue ,  NGUYEN, Bichlien

    IPC分类号： C12Q1/6806

    CPC分类号： C12Q1/6806 ,  G11C13/0019

    摘要： This disclosure describes a technique for performing random access in a pool of polynucleotides by using one unique primer and one homopolymer primer to selectively amplify some but not all of the polynucleotides in the pool. The polynucleotides are synthesized by a template independent polymerase such as terminal deoxynucleotide transferase (TdT) rather than by phosphoramidite synthesis. Enzymatic synthesis efficiently creates homopolymer sequences through unregulated synthesis. Use of one homopolymer primer instead of two unique primers decreases the complexity, time, and cost of synthesizing the polynucleotides. Use of a unique primer provides a sequence that can be varied to uniquely identify multiple different groups of polynucleotides. This enables random access by polymerase chain reaction (PCR) amplification while still benefitting from the efficiency of homopolymer synthesis. The polynucleotides may include payload regions that use a sequence of nucleotides to encode digital data.