公开(公告)号：EP1281712A1

公开(公告)日：2003-02-05

申请号：EP02255414.1

申请日：2002-08-01

申请人： Molecular Devices Corporation

发明人： Diwu, Zhenjun ,  Twu, Jesse J. ,  Yi, Guoliang ,  Lavis, Luke D. ,  Chen, Yen-Wen ,  Cassutt, Kelly J.

IPC分类号： C07D493/10 ,  C07D311/82 ,  G01N33/84 ,  C12Q1/44

CPC分类号： C07D493/10 ,  C07D311/82 ,  G01N1/30 ,  G01N33/84 ,  G01N2001/302 ,  G01N2800/04

摘要： Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2',7'-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds of the formula shown below. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

摘要翻译： 用于测量pH的系统，包括组合物和方法，特别是在细胞，细胞器和其它样品中。 组合物包括pH敏感的荧光和荧光2'，7'-二烷基荧光素衍生物和下列所示式的相关非荧光前体化合物。 组合物可以允许在激发光谱和发射光谱中进行比例测量。 所述方法包括向样品池中加入前体化合物，孵育样品池以释放游离指示剂，照射样品池，以及检测游离指示剂的荧光响应。