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13 条记录 (耗时 0.016 秒)

    A METHOD OF REGULATING OLIGONUCLEOTIDE FUNCTIONALITY
    2.
    发明公开
    A METHOD OF REGULATING OLIGONUCLEOTIDE FUNCTIONALITY 审中-公开
    一种调节寡核苷酸功能的方法

    公开(公告)号：EP2553124A1

    公开(公告)日：2013-02-06

    申请号：EP11761814.0

    申请日：2011-02-25

    申请人： Monoquant Pty Ltd ,  Flinders University

    发明人： MORLEY, Alexander, Alan ,  BRISCO, Michael, Julian

    IPC分类号： C12Q1/68

    CPC分类号： C12P19/34 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2527/107 ,  C12Q2549/119

    摘要： The present invention relates generally to a method of regulating oligonucleotide functionality and, more particularly, to a method of regulating the functionality of a primer or probe. The method of the invention is designed to provide a means to selectively inactivate or activate the functionality of an oligonucleotide, such as a primer, thereby providing means to regulate the progress of any method using that oligonucleotide. The development of a means to regulate the functionality of an oligonucleotide, such as a primer, is useful in a range of applications including, but not limited to, amplification reactions such as PCR, isothermal amplification and nucleic acid strand extension. With respect to amplification reactions, these have wide utility including the diagnosis and/or monitoring of disease conditions which are characterised by specific gene sequences and the characterisation or analysis of specific gene regions of interest.

    摘要翻译： 本发明一般涉及调节寡核苷酸功能的方法，并且更具体地涉及调节引物或探针的功能的方法。 设计本发明的方法以提供选择性失活或激活寡核苷酸（例如引物）的功能性的手段，由此提供调节使用该寡核苷酸的任何方法的进展的手段。 调节寡核苷酸（例如引物）功能的手段的开发可用于一系列应用，包括但不限于扩增反应如PCR，等温扩增和核酸链延伸。 就扩增反应而言，这些方法具有广泛的实用性，包括诊断和/或监测以特定基因序列为特征的疾病状况以及特定目的基因区域的表征或分析。

    A METHOD OF NUCLEIC ACID AMPLIFICATION
    3.
    发明公开
    A METHOD OF NUCLEIC ACID AMPLIFICATION 有权
    PROCEDURE用于扩增核酸

    公开(公告)号：EP2438184A1

    公开(公告)日：2012-04-11

    申请号：EP10782824.6

    申请日：2010-06-02

    申请人： Monoquant PTY LTD ,  Flinders University

    发明人： MORLEY, Alexander, Alan ,  BRISCO, Michel Julian

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2549/119 ,  C12Q2527/107

    摘要： The present invention relates generally to a method of amplifying a nucleic acid region of interest and, more particularly, to a method of amplifying a nucleic acid region of interest via a nested single tube PCR. The method of the invention is designed to provide a means to selectively inactivate the functionality of the outer primer or primers via an antisense nucleic acid molecule targeted to at least one of the outer primers, thereby maintaining amplification efficiency throughout the reaction. The development of a means to achieve efficient amplification by the outer primer followed by efficient amplification with the inner primers, in the context of a single tube nested PCR, is useful in a range of applications including, but not limited to, the diagnosis and/or monitoring of disease conditions which are characterised by specific gene sequences and the characterisation or analysis of specific gene regions of interest. Still further, the method of the present invention enables quantification to be performed and not just simple detection.

    A METHOD FOR DNA BREAKPOINT ANALYSIS
    5.
    发明公开
    A METHOD FOR DNA BREAKPOINT ANALYSIS 有权
    一种DNA断点分析方法

    公开(公告)号：EP2150628A1

    公开(公告)日：2010-02-10

    申请号：EP08756871.3

    申请日：2008-05-30

    申请人： Monoquant PTY LTD

    发明人： MORLEY, Alexander, Alan

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6858 ,  C12Q2549/119 ,  C12Q2537/143 ,  C12Q2525/155

    摘要： The present invention relates to a method for identifying a DNA breakpoint and agents for use therein. More particularly, the present invention provides a method for identifying a gene translocation breakpoint based on the application of a novel multiplex DNA amplification technique. The method of the present invention facilitates not only the identification of the breakpoint position but, further, enables the isolation of the DNA segment across which the breakpoint occurs. This provides a valuable opportunity to conduct further analysis of the breakpoint region, such as to sequence across this region. The method of the present invention is useful in a range of applications including, but not limited to, providing a routine means to characterise the gene breakpoint associated with disease onset in a patient and thereby enable the design of patient specific probes and primers for ongoing monitoring of the subject disease condition. In addition to monitoring the progression of a condition characterised by the existence of the breakpoint, there is also enabled assessment of the effectiveness of existing therapeutic drugs and/or new therapeutic drugs and, to the extent that the condition is a neoplasm, prediction of the likelihood of a subject's relapse from a remissive state.

    摘要翻译： 本发明涉及鉴定DNA断裂点的方法和其中使用的试剂。 更具体地说，本发明提供了基于应用新的多重DNA扩增技术来鉴定基因易位断点的方法。 本发明的方法不仅便于识别断点位置，而且便于分离出现断点的DNA片段。 这为断点区域的进一步分析提供了一个宝贵的机会，例如在整个区域进行排序。 本发明的方法在一系列应用中是有用的，包括但不限于提供表征与患者中疾病发作相关的基因断裂点的常规方法，从而能够设计用于持续监测的患者特异性探针和引物 的主题疾病状况。 除了监测以断裂点存在为特征的疾病的进展之外，还能够评估现有治疗药物和/或新治疗药物的有效性，并且在条件是肿瘤的情况下，可以预测 受试者从缓慢状态复发的可能性。

    A METHOD OF DETERMINING RNA INTEGRITY
    7.
    发明公开
    A METHOD OF DETERMINING RNA INTEGRITY 有权
    方法用于确定RNA的完整性

    公开(公告)号：EP2841602A1

    公开(公告)日：2015-03-04

    申请号：EP13782246.6

    申请日：2013-04-23

    申请人： Monoquant Pty Ltd

    发明人： MORLEY, Alexander, Alan ,  BRISCO, Michael, Julian

    IPC分类号： C12Q1/68

    CPC分类号： G06F19/20 ,  C12Q1/6809 ,  C12Q1/6851 ,  C12Q1/6886 ,  C12Q2537/165 ,  G06F19/10 ,  C12Q2525/204

    摘要： A method of determining a quantitative measure of the integrity of RNA in a sample, the method comprising: (i) assaying a sample containing instances of an RNA molecule transcribed from a reference gene, at least some of the instances being damaged, to determine quantitative measures of the relative or absolute numbers of intact instances of each of a plurality of segments of the RNA molecule in the sample, the segments having respective different lengths; (ii) based on a relationship between the determined quantitative measures and the respective different lengths of the segments, determining a quantitative measure of integrity of the instances of the RNA molecule in the sample; and (iii) determining the total number of instances of an RNA molecule of interest in a sample by using the quantitative measure of integrity and the length of a corresponding degradation-relevant segment of the RNA molecule of interest.