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    Stabilization of L-phenylalanine ammonia-lyase enzyme
    1.
    发明公开
    Stabilization of L-phenylalanine ammonia-lyase enzyme 失效
    L-苯丙氨酸氨基酸酶的稳定性

    公开(公告)号：EP0136996A3

    公开(公告)日：1987-04-01

    申请号：EP84870129

    申请日：1984-09-04

    申请人： Monsanto Company

    发明人： Kishore, Ganesh Murphy

    IPC分类号： C12N09/88 ,  C12N09/96 ,  C12P13/22 ,  C12N11/10

    CPC分类号： C12N11/10 ,  C12N9/88 ,  C12N9/96 ,  C12P13/222

    摘要： A process for the synthesis of L-phenylalanines and analogues thereof from trans-cinnamate, ring substituted trans-cinnamates or various acrylate derivatives and ammonia or an ammonia donor in the presence of L-phenylalanine ammonia-lyase enzyme and a desensitizing agent such as polyhydric alcohols and polyethylene glycol-(400) is disclosed. The process embraces the discovery that compounds such as polyhydric alcohols and polyethylene glycol-(400) desensitize the L-phenyalanine ammonia-lyase enzyme to substantially higher substrate concentrations than practiced heretofore. In addition, polyhydric alcohols and polyethylene glycol-(400) substantially enhance the instantaneous rate of reaction and inhibit inactivation of the L-phenylalanine ammonia lyase enzyme over longer reaction periods.

    Stabilization of L-phenylalanine ammonia-lyase enzyme
    3.
    发明公开
    Stabilization of L-phenylalanine ammonia-lyase enzyme 失效
    L-苯丙氨酸解氨酶的酶的稳定化。

    公开(公告)号：EP0136996A2

    公开(公告)日：1985-04-10

    申请号：EP84870129.8

    申请日：1984-09-04

    申请人： Monsanto Company

    发明人： Kishore, Ganesh Murphy

    IPC分类号： C12N9/88 ,  C12N9/96 ,  C12P13/22 ,  C12N11/10

    CPC分类号： C12N11/10 ,  C12N9/88 ,  C12N9/96 ,  C12P13/222

    摘要： A process for the synthesis of L-phenylalanines and analogues thereof from trans-cinnamate, ring substituted trans-cinnamates or various acrylate derivatives and ammonia or an ammonia donor in the presence of L-phenylalanine ammonia-lyase enzyme and a desensitizing agent such as polyhydric alcohols and polyethylene glycol-(400) is disclosed. The process embraces the discovery that compounds such as polyhydric alcohols and polyethylene glycol-(400) desensitize the L-phenyalanine ammonia-lyase enzyme to substantially higher substrate concentrations than practiced heretofore. In addition, polyhydric alcohols and polyethylene glycol-(400) substantially enhance the instantaneous rate of reaction and inhibit inactivation of the L-phenylalanine ammonia lyase enzyme over longer reaction periods.